Nerve growth factor increases electrical activity of neural cells derived from murine bone marrow stromal cells.
Nerve growth factor increases electrical activity of neural cells derived from murine bone marrow stromal cells.
复制标题
神经生长因子可增加源自小鼠骨髓基质细胞的神经细胞的电活动。
DOI：
--
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
K. Satomura
中科院分区：
文献类型：
--
作者：
S. Tomioka;T. Nagahama;Reiko Tokuyama;S. Tatehara;K. Satomura
客观化
神经生长因子(NGF)可触发神经元的长期兴奋性。我们检测了它对小鼠骨髓基质细胞(BMSC)来源神经元的影响。
方法
在优化的分化方案下，BMSCs在培养中被诱导分化为神经元。为证实骨髓间充质干细胞向神经元分化的可能性，用免疫细胞化学方法检测神经元标志蛋白神经丝的表达。为了研究BMSC来源神经元的电生理特性，采用细胞外记录技术记录未经处理(对照)的BMSC来源神经元和经NGF处理后的BMSC来源神经元的场电位。
结果
大多数骨髓间充质干细胞来源的神经元表现为自发放电，其幅度可达2 mV。在骨髓间充质干细胞来源的神经元中加入100 ng/ml的NGF后，离散场电位的幅值在1min内逐渐增大，3min后达到峰值(是对照组的20倍)。而应用NGF后5min，场电位幅值的增大几乎消失。
结论
这一发现表明，小鼠骨髓间充质干细胞来源的神经元自发地产生离散的场电位活动，而神经生长因子具有扩大骨髓间充质干细胞来源神经元的瞬时电活动而不是持续性电活动的作用。
OBJECTIVE
Nerve growth factor (NGF) triggers long-term neuronal excitability. We examined its effect on murine bone marrow stromal cells (BMSC)-derived neurons.
METHODS
With an optimal differentiation protocol, BMSCs were differentiated into neurons in culture. To confirm the probability of differentiation of BMSC into neuron, the expression of neuronal marker protein, neurofilament, was examined by immunocytochemistry. To examine the electrophysiological properties of BMSC-derived neurons, the field potentials were recorded either from nontreated (control) BMSC-derived neurons or from BMSC-derived neurons after the treatment with NGF by using extracellular recording techniques.
RESULTS
Most BMSC-derived neurons showed spontaneous discharges whose amplitudes were up to 2 mV. When NGF at a concentration of 100 ng/ml was applied to BMSC-derived neurons, the amplitudes of discrete field potentials were gradually enlarged within 1 min after NGF application and peaked 3 min later (20-fold the size of control). However, the enlargement of the amplitudes of field potentials almost disappeared 5 min after NGF application.
CONCLUSION
This finding indicates that neuronal cells derived from murine BMSCs generate discrete field potential activities spontaneously and that NGF has the effect of enlarging transient, but not sustained, electrical activity of BMSC-derived neurons.
PC12 细胞中的神经生长因子会急剧增加电压敏感的钙电流。
影响因子：
2.5
作者：
Jia,M;Li,M;Liu,XW;Jiang,H;Nelson,PG;Guroff,G
通讯作者：
Guroff,G
影响因子：
2.3
作者：
Black, IB;Woodbury, D
通讯作者：
Woodbury, D