A genome-wide genetic map of NB-LRR disease resistance loci in potato.
A genome-wide genetic map of NB-LRR disease resistance loci in potato.
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DOI：
10.1007/s00122-011-1602-z
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发表时间：
2011-08
影响因子：
5.4
通讯作者：
Goverse, Aska
中科院分区：
文献类型：
--
作者：
Bakker, Erin;Borm, Theo;Prins, Pjotr;van der Vossen, Edwin;Uenk, Gerda;Arens, Marjon;de Boer, Jan;van Eck, Herman;Muskens, Marielle;Vossen, Jack;van der Linden, Gerard;van Ham, Roeland;Klein-Lankhorst, Rene;Visser, Richard;Smant, Geert;Bakker, Jaap;Goverse, Aska
像所有的植物一样，马铃薯已经进化出了一个监视系统，该系统由大量编码免疫受体的基因组成，这些基因赋予了马铃薯对病原体和害虫的抵抗力。这些所谓的抗性或R蛋白中的大多数属于含有核苷酸结合和亮氨酸丰富重复结构域（NB-LRR）的超级家族。利用保守NB结构域的序列信息，研究了马铃薯NB- lrr抗性基因位点的全基因组遗传分布。对马铃薯二倍体无性系RH89-039-16 （S. tuberosum ssp）的BAC文库筛选得到的288个独特的BAC克隆进行了序列分析。tuberosum)和该BAC图书馆的物理地图。结果鉴定出738条部分和全长NB-LRR序列。根据这些序列与已知抗性基因的同源性，将280条和448条序列分别归类为TIR-NB-LRR （TNL）和CC-NB-LRR （CNL）序列。遗传图谱显示有15个TNL位点和32个CNL位点。其中36个是新发现的，而3个TNL位点和8个CNL位点与先前鉴定的功能性抗性基因是同源的。该遗传图谱与已有文献报道的68个通用CAPS标记和82个抗病性状位点相补充，为马铃薯遗传研究和应用研究提供了良好的模板。本文的在线版本（doi:10.1007/s00122-011-1602-z）包含补充材料，仅供授权用户使用。
Like all plants, potato has evolved a surveillance system consisting of a large array of genes encoding for immune receptors that confer resistance to pathogens and pests. The majority of these so-called resistance or R proteins belong to the super-family that harbour a nucleotide binding and a leucine-rich-repeat domain (NB-LRR). Here, sequence information of the conserved NB domain was used to investigate the genome-wide genetic distribution of the NB-LRR resistance gene loci in potato. We analysed the sequences of 288 unique BAC clones selected using filter hybridisation screening of a BAC library of the diploid potato clone RH89-039-16 (S. tuberosum ssp. tuberosum) and a physical map of this BAC library. This resulted in the identification of 738 partial and full-length NB-LRR sequences. Based on homology of these sequences with known resistance genes, 280 and 448 sequences were classified as TIR-NB-LRR (TNL) and CC-NB-LRR (CNL) sequences, respectively. Genetic mapping revealed the presence of 15 TNL and 32 CNL loci. Thirty-six are novel, while three TNL loci and eight CNL loci are syntenic with previously identified functional resistance genes. The genetic map was complemented with 68 universal CAPS markers and 82 disease resistance trait loci described in literature, providing an excellent template for genetic studies and applied research in potato. The online version of this article (doi:10.1007/s00122-011-1602-z) contains supplementary material, which is available to authorized users.
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