Pleiotropic Functions of High Fat Diet in the Etiology of Osteoarthritis.
Pleiotropic Functions of High Fat Diet in the Etiology of Osteoarthritis.
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DOI：
10.1371/journal.pone.0162794
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Okawa A
中科院分区：
文献类型：
--
作者：
Asou Y;Iwata M;Ochi H;Ailixiding M;Aibibula Z;Piao J;Jin G;Hara Y;Okawa A
肥胖是骨关节炎(OA)的危险因素。为了研究机械负荷增加在肥胖诱导的骨关节炎发病中的作用，将尾部悬吊(TS)模型应用于高脂饮食(HFD)诱导的骨关节炎模型后，对膝关节进行了组织学分析。将小鼠分为正常饮食(ND)+正常负荷(NL)组、HFD+正常负荷组、ND+TS组、HFD+TS组。采用免疫组织化学方法对全膝关节进行评价。摘除髌下脂肪垫(IPFP)，用定量聚合酶链式反应(QPCR)分析比较其基因表达谱。饮食12周后，NL组和TS组大鼠体重均明显增加。组织学观察，仅NL+HFD组关节软骨表层不规则。尽管TS+HFD组的骨赘面积小于NL+HFD组，但NL组和TS组的骨赘面积均因HFD而增加。在定量聚合酶链式反应对IPFP的评估中，脂肪因子和炎性细胞因子也因HFD而增加。而转化生长因子-β在高脂饮食组升高，TS组略低，与骨赘面积平行。采用原位末端标记法检测关节软骨细胞的凋亡率。无论机械载荷大小，HFD小鼠关节软骨内均可见大量TUNEL阳性细胞。由于HFD的结果，即使在TS组也可以看到IPFP炎症、软骨细胞凋亡增强和骨赘形成。总之，这些数据表明，HFD通过机械负荷依赖和独立的机制促进了骨赘的形成。
Obesity is a risk factor for osteoarthritis (OA). To investigate the roles of increased mechanical loading in the onset of obesity-induced OA, knee joints were histologically analyzed after applying a tail suspension (TS) model to a high-fat diet (HFD)-induced OA model. Mice were divided into four groups: normal diet (ND) with normal loading (NL) group; HFD with NL group; ND with TS group; and HFD with TS group. Whole knee joints were evaluated by immunohistological analysis. The infrapatellar fat pad (IPFP) was excised and mRNA expression profiles were compared by qPCR analysis. After twelve weeks of the diet, body weight was increased by HFD in both the NL group and TS group. Upon histological analysis, the irregularity of the surface layer of articular cartilage was observed only in the NL+HFD group. Osteophyte area increased as a result of HFD in both the NL and TS groups, although osteophyte area in the TS+HFD group was smaller than that of the NL+HFD group. In the evaluation of the IPFP by qPCR, adipokines and inflammatory cytokines also increased as a result of HFD. While TGF-β increased as a result of HFD, the trend was slightly lower in the TS group, in parallel with osteophyte area. To detect apoptosis of articular chondrocytes, TUNEL staining was employed. TUNEL-positive cells were abundantly observed in the articular cartilage in the HFD mice regardless of mechanical loading. IPFP inflammation, enhanced chondrocyte apoptosis, and osteophyte formation were seen even in the TS group as a result of a HFD. In all, these data demonstrate that HFD contributed to osteophyte formation through mechanical loading dependent and independent mechanisms.
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DOI：
10.1084/jem.193.3.399
发表时间：
2001-02-05
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Ishijima M;Rittling SR;Yamashita T;Tsuji K;Kurosawa H;Nifuji A;Denhardt DT;Noda M
通讯作者：
Noda M
DOI：
10.1258/mi.2008.008018
发表时间：
2008-12-01
期刊：
Menopause international
影响因子：
--
作者：
Magliano, Malgorzata
通讯作者：
Magliano, Malgorzata
影响因子：
7
作者：
Säämänen, AMK;Salminen, HJ;Metsäranta, MPH
通讯作者：
Metsäranta, MPH
影响因子：
27.4
作者：
Bastiaansen-Jenniskens, Yvonne M.;Clockaerts, Stefan;van Osch, Gerjo J. V. M.
通讯作者：
van Osch, Gerjo J. V. M.
影响因子：
27.4
作者：
Dahaghin, S.;Bierma-Zeinstra, S. M. A.;Pols, H. A. P.
通讯作者：
Pols, H. A. P.