Practical application of monoclonal antibodies to the diagnosis and classification of acute leukaemias.
Practical application of monoclonal antibodies to the diagnosis and classification of acute leukaemias.
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单克隆抗体在急性白血病诊断和分类中的实际应用。
DOI：
10.1111/j.1365-2257.1987.tb00087.x
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Borowitz,MJ
中科院分区：
文献类型：
--
作者：
Wain,SL;Borowitz,MJ
急性白血病患者在预后和治疗反应方面表现为一个不同的群体。过去，髓系白血病和淋巴系白血病的鉴别主要基于恶性细胞的形态和组织化学分析。用多克隆抗血清和绵羊红细胞花环技术将淋巴白血病亚型分为T细胞(E玫瑰花结+)、B细胞(Sig+)和前B细胞(细胞质Ig+)，取得了进一步的进展。随后，针对其他淋巴细胞或白血病相关抗原的异源抗血清扩大了我们对白血病多样性的认识。然而，抗体试剂的可变的特异性和有限的可用性，以及标准化玫瑰花环试验的困难，使得在临床实验室进行白血病的常规表型鉴定是不可行的。单抗技术的发展提供了大规模生产和标准化试剂广泛可用的优势(Kohler&Milstein，1975)。此外，由单抗定义的大量分化抗原对进一步完善急性白血病的分类是有用的。制造单抗的最初策略是生产检测白血病细胞选择性表达的糖蛋白、糖脂或碳水化合物的试剂。然而，到目前为止生产的所有抗体都与非白血病细胞特有的抗原发生了反应。在许多情况下，这些抗原与正常的淋巴细胞分化阶段有关，它们在白血病细胞上的存在表明，白血病细胞在特定的分化阶段被阻断。这一假设绝不是被普遍接受的。然而，通过将白血病与已知的正常生理学联系起来，就有可能对白血病表型的复杂性提出更一致的看法。正因为如此，本审查将尽可能采用这种方法。举办了三个关于人类白细胞分化抗原的国际研讨会，并发表了其中两个研讨会的摘要(Reinherz，Haynes&Nadler，1986；Bernard等人)。1984年)。至少400种针对T淋巴细胞、B淋巴细胞和髓系细胞表面结构的抗体-
Patients with acute leukaemia represent a heterogeneous group with regard to prognosis and response to therapy. In the past, distinction between leukaemias of myeloid and lymphoid origin was based mainly on morphologic and histochemical analysis of the malignant cells. Further advances were made when lymphoid leukaemias were subclassified into T-cell (E rosette+), B-cell (SIg+) and later, pre-B-cell (cytoplasmic Ig+) types using polyclonal antisera and sheep red cell rosetting techniques. Subsequently, heteroantisera against other lymphocyte or leukaemia associated antigens expanded our knowledge of leukaemic diversity. However, the variable specificity and limited availability of the antibody reagents and the difficulties of standardizing the rosetting assays made routine phenotyping of leukaemia in the clinical laboratory impractical. The development of monoclonal antibody technology offers the advantages of large scale production and widespread availability of standardized reagents (Kohler & Milstein 1975). Moreover, the large number of differentiation antigens defined by monoclonal antibodies have been useful in further refining the classification of acute leukaemias.The initial strategy in making monoclonal antibodies was to produce reagents which detect glycoproteins, glycolipids or carbohydrates selectively expressed by leukaemic cells. However, all antibodies produced to date have reacted with antigens that have not been specific for leukaemic cells. In many cases, these antigens have been associated with a stage of normal lymphocyte differentiation, and their presence on leukaemic cells has suggested as a hypothesis that leukaemic cells are blocked at a particular stage of differentiation. This hypothesis is by no means universally accepted. By relating leukaemias to what is known about normal physiology, however, it is possible to present a more coherent picture of the complexity of leukaemia phenotyping. It is for this reason that such an approach will be used where possible in this review. Three International Workshops on Human Leukocyte Differentiation Antigens have been held and summaries of two of them published (Reinherz, Haynes & Nadler 1986; Bernard et al. 1984). At least 400 antibodies directed against surface structures on T-lymphocytes, B-lymphocytes, and myeloid-
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用于检测 T 细胞前体白血病 (T-ALL) 的单克隆抗体 (WT1)。
影响因子：
20.3
作者：
L. Vodinelich;W. Tax;Y. Bai;S. Pegram;P. Capel;M. Greaves
通讯作者：
M. Greaves
影响因子：
20.3
作者：
Strauss,LC;Stuart,RK;Civin,CI
通讯作者：
Civin,CI
TdT 阳性急性淋巴细胞白血病细胞上骨髓标记物的表达：双荧光染色证据。
影响因子：
20.3
作者：
P. Bettelheim;E. Paietta;O. Majdic;H. Gadner;J. Schwarzmeier;W. Knapp
通讯作者：
W. Knapp
T 细胞受体 β 链基因重排：T 细胞谱系和克隆性的遗传标记。
影响因子：
3.3
作者：
Knowles2nd,DM;Pelicci,PG;Dalla-Favera,R
通讯作者：
Dalla-Favera,R
DOI：
--
发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Dimitriu-Bona,A;Burmester,GR;Waters,SJ;Winchester,RJ
通讯作者：
Winchester,RJ