Changes of Small Non-coding RNAs by Severe Acute Respiratory Syndrome Coronavirus 2 Infection.
Changes of Small Non-coding RNAs by Severe Acute Respiratory Syndrome Coronavirus 2 Infection.
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DOI：
10.3389/fmolb.2022.821137
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发表时间：
2022
影响因子：
5
通讯作者：
Bao X
中科院分区：
文献类型：
--
作者：
Wu W;Choi EJ;Wang B;Zhang K;Adam A;Huang G;Tunkle L;Huang P;Goru R;Imirowicz I;Henry L;Lee I;Dong J;Wang T;Bao X
由于严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)的快速传播，正在进行的2019年冠状病毒病(新冠肺炎)大流行是一个重大的全球公共卫生威胁，其发病机制基本未知。在病毒感染的背景下，小的非编码RNA(SncRNAs)在调节宿主反应、病毒复制和宿主-病毒相互作用方面发挥着重要作用。与包括microRNAs(MiRNAs)和Piwi相互作用RNAs(PiRNAs)在内的其他SncRNAs亚家族相比，tRNA衍生的RNA片段(TRFs)相对较新，是宿主与病毒相互作用的重要调节因子。利用一种改进的下一代测序方法(NGS)T4PNK-RNA-seq，我们发现SARS-CoV-2对人鼻咽拭子(NPS)样本中的SncRNA谱有显著影响。在受影响的SncRNAs中，受影响最大的是TRFs，它们大多来源于tRNAs的5‘端(TRF5)。在SARS-CoV-2感染的呼吸道上皮细胞中也观察到了这种变化。除了宿主来源的ncRNAs外，我们还发现了几个小的病毒来源的ncRNAs(SvRNAs)，其中来自CoV2基因组346-382位的svRNA(SV-CoV2-346)表达最高。TRFs和SV-CoV2-346的诱导以前没有报道，因为这些SncRNAs的3‘-OH端的缺乏使它们无法被常规的NGS检测到。综上所述，我们的研究证明了TRFs参与了新冠肺炎的表达，并揭示了新的CoV2svRNA。
The ongoing pandemic of coronavirus disease 2019 (COVID-19), which results from the rapid spread of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a significant global public health threat, with molecular mechanisms underlying its pathogenesis largely unknown. In the context of viral infections, small non-coding RNAs (sncRNAs) are known to play important roles in regulating the host responses, viral replication, and host-virus interaction. Compared with other subfamilies of sncRNAs, including microRNAs (miRNAs) and Piwi-interacting RNAs (piRNAs), tRNA-derived RNA fragments (tRFs) are relatively new and emerge as a significant regulator of host-virus interactions. Using T4 PNK‐RNA‐seq, a modified next-generation sequencing (NGS), we found that sncRNA profiles in human nasopharyngeal swabs (NPS) samples are significantly impacted by SARS-CoV-2. Among impacted sncRNAs, tRFs are the most significantly affected and most of them are derived from the 5′-end of tRNAs (tRF5). Such a change was also observed in SARS-CoV-2-infected airway epithelial cells. In addition to host-derived ncRNAs, we also identified several small virus-derived ncRNAs (svRNAs), among which a svRNA derived from CoV2 genomic site 346 to 382 (sv-CoV2-346) has the highest expression. The induction of both tRFs and sv-CoV2-346 has not been reported previously, as the lack of the 3′-OH ends of these sncRNAs prevents them to be detected by routine NGS. In summary, our studies demonstrated the involvement of tRFs in COVID-19 and revealed new CoV2 svRNAs.
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影响因子：
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作者：
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通讯作者：
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DOI：
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发表时间：
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影响因子：
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影响因子：
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作者：
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