Vascular Adhesion Protein-1 Determines the Cellular Properties of Endometrial Pericytes.
Vascular Adhesion Protein-1 Determines the Cellular Properties of Endometrial Pericytes.
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DOI：
10.3389/fcell.2020.621016
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发表时间：
2020
影响因子：
5.5
通讯作者：
Tan BK
中科院分区：
文献类型：
--
作者：
Gharanei S;Fishwick K;Peter Durairaj R;Jin T;Siamantouras E;Liu KK;Straube A;Lucas ES;Weston CJ;Rantakari P;Salmi M;Jalkanen S;Brosens JJ;Tan BK
血管粘附蛋白-1（VAP-1）是一种炎症诱导的粘附分子，也是一种参与免疫细胞运输的一级胺氧化酶。白细胞外渗到组织中是由内皮细胞和周细胞上表达的粘附分子介导的。周细胞在周期性子宫内膜的血管生成和血管化中起主要作用。然而，人子宫内膜周细胞的功能特性尚不清楚。在这里，我们发现，在黄体中期的人子宫内膜螺旋小动脉周围的周细胞组成性表达VAP-1。我们首先描述了这些周细胞的特征，并证明VAP-1的敲除扰乱了它们的生物物理特性，并损害了它们的收缩、迁移、粘附和克隆形成能力。此外，我们发现VAP-1的缺失破坏了体外周细胞-子宫自然杀伤细胞的相互作用。总之，这些数据不仅揭示了子宫内膜周细胞代表了具有不同生物物理和功能特性的细胞群体，而且还表明VAP-1在调节人类子宫内膜中先天免疫细胞的募集中起着关键作用。我们认为，VAP-1可以作为一个潜在的生物标志物，妊娠病理所造成的损害血管周围环境之前的概念。
Vascular adhesion protein-1 (VAP-1) is an inflammation-inducible adhesion molecule and a primary amine oxidase involved in immune cell trafficking. Leukocyte extravasation into tissues is mediated by adhesion molecules expressed on endothelial cells and pericytes. Pericytes play a major role in the angiogenesis and vascularization of cycling endometrium. However, the functional properties of pericytes in the human endometrium are not known. Here we show that pericytes surrounding the spiral arterioles in midluteal human endometrium constitutively express VAP-1. We first characterize these pericytes and demonstrate that knockdown of VAP-1 perturbed their biophysical properties and compromised their contractile, migratory, adhesive and clonogenic capacities. Furthermore, we show that loss of VAP-1 disrupts pericyte-uterine natural killer cell interactions in vitro. Taken together, the data not only reveal that endometrial pericytes represent a cell population with distinct biophysical and functional properties but also suggest a pivotal role for VAP-1 in regulating the recruitment of innate immune cells in human endometrium. We posit that VAP-1 could serve as a potential biomarker for pregnancy pathologies caused by a compromised perivascular environment prior to conception.
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