Conversion of the high-yield salinomycin producer Streptomyces albus BK3-25 into a surrogate host for polyketide production
Conversion of the high-yield salinomycin producer Streptomyces albus BK3-25 into a surrogate host for polyketide production
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将高产盐霉素生产者白色链霉菌 BK3-25 转化为聚酮化合物生产的替代宿主
DOI：
10.1007/s11427-017-9122-8
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发表时间：
2017-08
影响因子：
9.1
通讯作者：
Bai Linquan
中科院分区：
文献类型：
--
作者：
Zhang Xiaojie;Lu Chenyang;Bai Linquan
An ideal surrogate host for heterologous production of various natural products is expected to have efficient nutrient utilization, fast growth, abundant precursors and energy supply, and a pronounced gene expression. Streptomyces albus BK3-25 is a high-yield industrial strain producing type-I polyketide salinomycin, with a unique ability of bean oil utilization. Its potential of being a surrogate host for heterologous production of PKS was engineered and evaluated herein. Firstly, introduction of a three-gene cassette for the biosynthesis of ethylmalonyl-CoA resulted in accumulation of ethylmalonyl-CoA precursor and salinomycin, and subsequent deletion of the salinomycin biosynthetic gene cluster resulted in a host with rich supplies of common polyketide precursors, including malonyl-CoA, methylmalonyl-CoA, and ethylmalonyl-CoA. Secondly, the energy and reducing force were measured, and the improved accumulation of ATP and NADPH was observed in the mutant. Furthermore, the strength of a series of selected endogenous promoters based on microarray data was assessed at different growth phases, and a strong constitutive promoter was identified, providing a useful tool for further engineered gene expression. Finally, the potential of the BK3-25 derived host ZXJ-6 was evaluated with the introduction of the actinorhodin biosynthetic gene cluster from Streptomyces coelicolor, and the heterologous production of actinorhodin was obtained. This work clearly indicated the potential of the high-yield salinomycin producer as a surrogate host for heterologous production of polyketides, although more genetic manipulation should be conducted to streamline its performance.
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影响因子：
2.7
作者：
H. Shinkawa;Y. Hatada;M. Okada;H. Kinashi;O. Nimi
通讯作者：
H. Shinkawa;Y. Hatada;M. Okada;H. Kinashi;O. Nimi
影响因子：
5.2
作者：
Beites T;Mendes MV
通讯作者：
Mendes MV
影响因子：
56.9
作者：
KAO, CM;KATZ, L;KHOSLA, C
通讯作者：
KHOSLA, C
DOI：
10.7164/antibiotics.42.1768
发表时间：
1989-12
期刊：
The Journal of antibiotics
影响因子：
--
作者：
M. Hara;K. Asano;I. Kawamoto;T. Takiguchi;S. Katsumata;K. Takahashi;H. Nakano
通讯作者：
M. Hara;K. Asano;I. Kawamoto;T. Takiguchi;S. Katsumata;K. Takahashi;H. Nakano
影响因子：
4.4
作者：
Felnagle, Elizabeth A.;Rondon, Michelle R.;Thomas, Michael G.
通讯作者：
Thomas, Michael G.