Circulating microRNA: a novel potential biomarker for early diagnosis of intracranial aneurysm rupture a case control study.
Circulating microRNA: a novel potential biomarker for early diagnosis of intracranial aneurysm rupture a case control study.
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循环 microRNA：一种用于早期诊断颅内动脉瘤破裂的新型潜在生物标志物病例对照研究
DOI：
10.1186/1479-5876-11-296
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发表时间：
2013-11-27
影响因子：
7.4
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Jin H;Li C;Ge H;Jiang Y;Li Y
目的应用微阵列杂交技术检测颅内动脉瘤破裂患者血清miRNA水平，探讨其对颅内动脉瘤破裂的预警作用。动脉瘤合并子瘤组、动脉瘤不合并子瘤组、动脉瘤破裂组和血管造影阴性组。然后使用血清miRNA进行微阵列研究。鉴定了差异表达的miRNA。为了使结果看起来更直观，进行聚类分析，并从miRNA靶预测数据库检索潜在的基因targets.ResultsMicroarray研究确定了86个miRNAs的表达水平之间的显着差异（p < 0.05）的三个实验组和对照组。其中69个上调，17个下调。A组所有miRNA表达上调，B、C组各有上调和下调。共有8291个预测的靶基因与这些miRNAs相关。生物信息学分析显示，有几个靶基因参与了与血管壁功能相关的细胞凋亡和活化。结论基因水平的方法揭示了正常人和动脉瘤患者血清中以及动脉瘤不同阶段中存在的几种不同的miRNA，提示miRNA可能参与了颅内动脉瘤的发生和发展的调控。对颅内动脉瘤破裂也有警示作用。A组差异表达的miRNA均上调，提示miRNA对颅内血管壁具有保护作用。
ObjectiveTo investigate warning effect of serum miRNA for intracranial aneurysm rupture through microarray hybridization.Methods24 were selected from 560 patients in our department and divided into group A, B, C and D. They are aneurysms with daughter aneurysms group, aneurysm without daughter aneurysms group, ruptured aneurysms group and angiography negative group. Then a microarray study was carried out using serum miRNA. Differentially expressed miRNAs were identified. Cluster analysis was performed in order to make the results looks more intuitive and potential gene targets were retrieved from miRNA target prediction databases.ResultsMicroarray study identified 86 miRNAs with significantly different (p < 0.05) expression levels between three experimental groups and control group. Among them 69 are up-regulated and 17 are down-regulated. All miRNAs in group A are up-regulated, while there are up and down-regulated in group B and C. A total of 8291 predicted target genes are related to these miRNAs. Bioinformatic analysis revealed that several target genes are involved in apoptosis and activation of cells associated with function of vascular wall.ConclusionOur gene level approach reveals several different serum miRNAs between normal people and aneurysm patients, as well as among different phases of aneurysm, suggesting that miRNA may participate in the regulation of the occurrence and development of intracranial aneurysm, and also have warning effect for intracranial aneurysm rupture. All differently expressed miRNA in group A are up-regulated, which may suggesting protective function of miRNA for intracranial vascular wall.
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影响因子：
2.7
作者：
Nygaard S;Jacobsen A;Lindow M;Eriksen J;Balslev E;Flyger H;Tolstrup N;Møller S;Krogh A;Litman T
通讯作者：
Litman T
DOI：
10.1038/nrrheum.2010.81
发表时间：
2010-07
期刊：
Nature reviews. Rheumatology
影响因子：
--
作者：
通讯作者：
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胸主动脉夹层与正常胸主动脉之间的 microRNA 谱比较表明 microRNA 在胸主动脉夹层发病机制中的潜在作用
影响因子：
4.3
作者：
Liao, Mingfang;Zou, Sili;Jing, Zaiping
通讯作者：
Jing, Zaiping
DOI：
10.1073/pnas.0813371106
发表时间：
2009-03-17
影响因子：
11.1
作者：
Wang, Kai;Zhang, Shile;Galas, David J.
通讯作者：
Galas, David J.
影响因子：
37.8
作者：
Small EM;Frost RJ;Olson EN
通讯作者：
Olson EN