Digital transplantation pathology: combining whole slide imaging, multiplex staining and automated image analysis.
Digital transplantation pathology: combining whole slide imaging, multiplex staining and automated image analysis.
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DOI：
10.1111/j.1600-6143.2011.03797.x
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发表时间：
2012-01
期刊：
影响因子：
--
通讯作者：
Demetris AJ
中科院分区：
文献类型：
--
作者：
Isse K;Lesniak A;Grama K;Roysam B;Minervini MI;Demetris AJ
传统的组织病理学是监测同种异体移植物的黄金标准，但其价值主张越来越受到质疑。对组织、外周血液和液体的“OMICS”分析和有针对性的血清学研究为同种异体移植物损伤提供了传统组织学目前所不能提供的机制洞察。然而，显微活检分析提供了有价值的和独特的信息：a)时空关系；b)罕见事件/细胞；c)复杂的结构背景；d)融入“系统”模型。然而，除了免疫染色，在过去的100多年里，没有任何变革性的进步使常规显微镜“现代化”。病理学家现在与硬件和软件工程师合作，利用数字成像、纳米粒子多重染色和计算图像分析软件的显著发展，弥合传统组织学-全球“组学”分析的差距。包括并列比较、客观活检发现量化、多路传输、自动图像分析以及电子数据和资源共享。目前对教学、质量保证、会议、咨询、研究和临床试验的利用正在朝着实施小容量、高复杂性临床服务的方向发展，如移植病理学。成本、实施的复杂性、不断变化的标准以及悬而未决的医疗/法律和监管问题仍然是挑战。无论如何，挑战将被克服，这些技术将使移植病理学家能够增加从组织样本中提取信息，并为跨平台生物标记物发现做出贡献，以改善结果。
Conventional histopathology is the gold standard for allograft monitoring, but its value proposition is increasingly questioned. “-Omics” analysis of tissues, peripheral blood and fluids and targeted serologic studies provide mechanistic insights into allograft injury not currently provided by conventional histology. Microscopic biopsy analysis, however, provides valuable and unique information: a) spatial-temporal relationships; b) rare events/cells; c) complex structural context; and d) integration into a “systems” model. Nevertheless, except for immunostaining, no transformative advancements have “modernized” routine microscopy in over 100 years. Pathologists now team with hardware and software engineers to exploit remarkable developments in digital imaging, nanoparticle multiplex staining, and computational image analysis software to bridge the traditional histology - global “–omic” analyses gap. Included are side-by-side comparisons, objective biopsy finding quantification, multiplexing, automated image analysis, and electronic data and resource sharing. Current utilization for teaching, quality assurance, conferencing, consultations, research and clinical trials is evolving toward implementation for low-volume, high-complexity clinical services like transplantation pathology. Cost, complexities of implementation, fluid/evolving standards, and unsettled medical/legal and regulatory issues remain as challenges. Regardless, challenges will be overcome and these technologies will enable transplant pathologists to increase information extraction from tissue specimens and contribute to cross-platform biomarker discovery for improved outcomes.
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影响因子：
6.1
作者：
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影响因子：
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作者：
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