Cold induces micro- and nano-scale reorganization of lipid raft markers at mounds of T-cell membrane fluctuations.
Cold induces micro- and nano-scale reorganization of lipid raft markers at mounds of T-cell membrane fluctuations.
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寒冷诱导 T 细胞膜波动处脂筏标记物的微米级和纳米级重组
DOI：
10.1371/journal.pone.0005386
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Chen ZW
中科院分区：
文献类型：
--
作者：
Chen Y;Qin J;Cai J;Chen ZW
寒冷是否以及如何引起细胞膜或脂筏的变化仍然没有得到很好的描述。利用NSOM/QD和共聚焦成像系统，我们发现寒冷导致Jurkat T细胞上脂筏标志物GM1和蛋白质CD59从微域的外围到中心的微尺度重新分布，并导致脂筏标志物纳米团的纳米尺度扩大(1/3~2/3倍)，甚至GM1和CD59纳米团的共存。这些发现表明，冷诱导的RAFT相关的膜异质性的侧向重排/合并。在接近自然的条件下，低温诱导脂筏标志物的重新分布为它们的变化提供了线索，并有助于提出一种模型，在该模型中，脂筏脂类与蛋白质组分结合或相互作用，以产生响应刺激的功能膜异质性。这些数据还强调了早期描述的冷相关实验中可能存在的冷诱导伪影，以及在4℃下对脂筏进行的基于洗涤剂抗性的分析，并为最近报道的冷诱导T细胞信号通路激活提供了生物物理解释。重要的是，我们的荧光地形图NSOM成像显示，GM1/CD59 RAFT标记在T细胞膜波动的丘丘上分布和重新分布，而不是在T细胞膜波动的凹陷。这种堆积分布的脂筏标记或脂筏为脂筏或接触分子提供了空间优势，便于与邻近细胞或自由分子相互作用。
Whether and how cold causes changes in cell-membrane or lipid rafts remain poorly characterized. Using the NSOM/QD and confocal imaging systems, we found that cold caused microscale redistribution of lipid raft markers, GM1 for lipid and CD59 for protein, from the peripheral part of microdomains to the central part on Jurkat T cells, and that cold also induced the nanoscale size-enlargement (1/3- to 2/3-fold) of the nanoclusters of lipid raft markers and even the colocalization of GM1 and CD59 nanoclusters. These findings indicate cold-induced lateral rearrangement/coalescence of raft-related membrane heterogeneity. The cold-induced re-distribution of lipid raft markers under a nearly-natural condition provide clues for their alternations, and help to propose a model in which raft lipids associate themselves or interact with protein components to generate functional membrane heterogeneity in response to stimulus. The data also underscore the possible cold-induced artifacts in early-described cold-related experiments and the detergent-resistance-based analyses of lipid rafts at 4°C, and provide a biophysical explanation for recently-reported cold-induced activation of signaling pathways in T cells. Importantly, our fluorescence-topographic NSOM imaging demonstrated that GM1/CD59 raft markers distributed and re-distributed at mounds but not depressions of T-cell membrane fluctuations. Such mound-top distribution of lipid raft markers or lipid rafts provides spatial advantage for lipid rafts or contact molecules interacting readily with neighboring cells or free molecules.
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