Synthesis and microcontact printing of dual end-functionalized mucin-like glycopolymers for microarray applications.
Synthesis and microcontact printing of dual end-functionalized mucin-like glycopolymers for microarray applications.
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DOI：
10.1002/anie.200805756
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发表时间：
2009
影响因子：
16.6
通讯作者：
Bertozzi, Carolyn R.
中科院分区：
文献类型：
--
作者：
Godula, Kamil;Rabuka, David;Nam, Ki Tae;Bertozzi, Carolyn R.
糖阵列是日益流行的工具，用于探测糖结合受体、[1]抗体、[2]和酶的配基特异性。[1C，d，f，3]在典型的体系结构中，使用添加到糖S还原端的连接物将糖组分连接到阵列表面。表面显示的多价性可以在有限的程度上反映细胞表面的环境。其中，多糖被组织成多价集合，这对于高亲和力与同源受体的结合通常是至关重要的。[4]然而，细胞表面的多糖的空间排列肯定不同于合成的糖阵。在细胞上，许多多糖以三维几何形式显示在糖蛋白支架上，这是在二维底物上无法模拟的。人们普遍认识到，多糖的相对位置可以深刻地影响寡聚受体的识别。[5]此外，连接物长度、[1f]糖密度、[1D，6]和糖固定方式的变化[7]可以改变糖-蛋白质相互作用的亲和力和特异性。应该探索在阵列平台内展示多糖的新模式，以更好地接近天然糖共轭结构。
Glycan arrays are increasingly popular tools for probing the ligand specificities of glycan-binding receptors,[1] antibodies,[2] and enzymes.[1c, d, f, 3] In a typical architecture, the glycan components are attached to the array surface using a linker appended to the glycan s reducing end. The multivalency of surface display can mirror, to a limited extent, the environment of the cell surface. Therein, glycans are organized into multivalent collections that are often critical for high-avidity binding to cognate receptors.[4] However, the spatial arrangement of glycans on a cell surface is surely different from that on a synthetic glycoarray. On cells, many glycans are displayed on glycoprotein scaffolds with three-dimensional geometries that cannot be emulated on a two-dimensional substrate. It is widely appreciated that the relative positioning of glycans can profoundly influence recognition by oligomerized receptors.[5] Additionally, changes in linker length,[1f] glycan density,[1d, 6] and mode of glycan immobilization can [7] alter the avidity and specificity of glycan–protein interactions. New modes of glycan display within array platforms should be explored to better approximate native glycoconjugate structures.
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