Nascent Proteins Caught in the Act
Nascent Proteins Caught in the Act
复制标题
被捕的新生蛋白质
作者：
M. Kampmann;G. Blobel
冷冻电子显微镜结构可视化两种类型的新生多肽链，影响蛋白质跨膜转运或细菌基因表达的调控。核糖体、相关翻译因子和转运RNA（tRNA）的X射线晶体学快照已经允许重建蛋白质翻译的动态方面。仍然缺少的是对事件的详细结构理解，以及翻译。在这个问题上的两个里程碑式的论文（1，2）使用冷冻电子显微镜（cryoEM），以获得亚纳米分辨率的信息，使新生的多肽链在核糖体隧道的直接可视化。核糖体-新生链复合物（RNCs）的两种不同的新生链指示两种不同的下游途径。
Cryo–electron microscopy structures visualize two types of nascent polypeptide chains that affect either protein translocation across membranes or regulation of bacterial gene expression. X-ray crystallographic snapshots of ribosomes, associated translation factors, and transfer RNA (tRNA) have allowed dynamic aspects of protein translation to be reconstructed. What is still missing is a detailed structural understanding of events coupled to translation. Two landmark papers in this issue (1, 2) use cryo–electron microscopy (cryoEM) to obtain information at subnanometer resolution, enabling the direct visualization of nascent polypeptide chains in the ribosomal tunnel. The two different nascent chains of ribosome-nascent chain complexes (RNCs) instruct two distinct downstream pathways.
DOI：
10.1126/science.1177662
发表时间：
2009-12-04
期刊：
Science (New York, N.Y.)
影响因子：
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作者：
Seidelt B;Innis CA;Wilson DN;Gartmann M;Armache JP;Villa E;Trabuco LG;Becker T;Mielke T;Schulten K;Steitz TA;Beckmann R
通讯作者：
Beckmann R