Polyion selective polymeric membrane-based pulstrode as a detector in flow-injection analysis.
Polyion selective polymeric membrane-based pulstrode as a detector in flow-injection analysis.
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DOI：
10.1021/ac500567g
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发表时间：
2014-04-15
影响因子：
7.4
通讯作者：
Meyerhoff, Mark E.
中科院分区：
文献类型：
--
作者：
Bell-Vlasoy, Andrea K.;Zajda, Joanna;Eldourghamy, Ayman;Malinowska, Elzbieta;Meyerhoff, Mark E.
本文研究了在流动注射分析系统中，用完全可逆的聚离子选择性聚合膜型脉冲振荡器作为检测器检测聚离子的方法。检测电极由掺杂有10重量%的三十二烷基甲基铵-二壬基萘磺酸盐（TDMA/DNNS）离子交换盐的增塑聚合物膜组成。所用的脉冲序列包括短（1 s）恒电流脉冲、测量电池EMF的开路脉冲（0.5s）和使膜返回其原始化学组成的较长（15 s）恒电位脉冲。研究表明，可以优化总脉冲序列时间，以高达20个样本/小时的速度对鱼精蛋白和肝素的注射样本进行可重复的实时检测。此外，它表明，相同的膜检测器可以用于FIA检测水平≥10 μg/mL的聚阳离子和水平≥40 μg/mL的聚阴离子，通过改变恒电流脉冲的方向。所描述的方法也可适用于在其他流动配置中检测低水平的聚离子物质，例如在液相色谱和毛细管电泳中。
A method for the detection of polyions using fully reversible polyion selective polymeric membrane type pulstrodes as detectors in a flow-injection analysis (FIA) system is examined. The detection electrode consists of a plasticized polymeric membrane doped with 10 wt % of tridodecylmethylammonium-dinonylnaphthalene sulfonate (TDMA/DNNS) ion-exchanger salt. The pulse sequence used involves a short (1 s) galvanostatic pulse, an open-circuit pulse (0.5 s) during which the EMF of the cell is measured, and a longer (15 s) potentiostatic pulse to return the membrane to its original chemical composition. It is shown that total pulse sequence times can be optimized to yield reproducible real-time detection of injected samples of protamine and heparin at up to 20 samples/h. Further, it is shown that the same membrane detector can be employed for FIA detection of both polycations at levels ≥10 μg/mL and polyanions at levels of ≥40 μg/mL by changing the direction of the galvanostatic pulse. The methodology described may also be applicable in the detection of polyionic species at low levels in other flowing configurations, such as in liquid chromatography and capillary electrophoresis.
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