Ecological Restoration of Antibiotic-Disturbed Gastrointestinal Microbiota in Foregut and Hindgut of Cows.
Ecological Restoration of Antibiotic-Disturbed Gastrointestinal Microbiota in Foregut and Hindgut of Cows.
复制标题
抗生素干扰的奶牛前肠和后肠胃肠道微生物群的生态恢复
DOI：
10.3389/fcimb.2018.00079
复制
发表时间：
2018
影响因子：
5.7
通讯作者：
Li S
中科院分区：
文献类型：
--
作者：
Ji S;Jiang T;Yan H;Guo C;Liu J;Su H;Alugongo GM;Shi H;Wang Y;Cao Z;Li S
抗生素引起的胃肠道菌群失调需要较长的时间才能恢复正常，这可能会给宿主带来一系列问题。通过抗生素恢复有偏见的微生物群的理解在很大程度上仍然是未知的。在这里，我们研究了抗生素暴露后以及抗生素戒断后（微生物群移植，MT组）或无（对照，CON组）微生物群移植的泌乳奶牛前肠（瘤胃）和后肠（直肠）中的微生物群变化。我们能够证明，在抗生素暴露3或14天后，前肠和后肠的微生物群都发生了显著变化，并且在停用抗生素后的很长一段时间内（>18天），这些变化持续存在。我们进一步观察到MT组前肠和后肠微生物群的恢复比CON组更快，前肠微生物群主要通过恢复α多样性以及组内相似性而受益于微生物群移植，而后肠微生物群主要通过重建共现网络而受益于微生物群移植（节点数、边数、密度、模块度以及接近中心度）。这些结果共同扩展了我们对抗生素恢复偏倚微生物群的理解，并且也可能对处理至少在奶牛中延迟的微生物群恢复具有指导意义。
Antibiotically disturbed gastrointestinal microbiota needs a long period time to be restored to normal, which may cause a series of problems to the host. The understanding of restoration of the biased microbiota by antibiotics remains largely unknown. Here, we investigated the microbiota shift in foregut (rumen) and hindgut (rectum) of lactating cows after antibiotics exposure as well as after antibiotics withdrawal with (Microbiota transplantation, MT group) or without (Control, CON group) microbiota transplantation. We were able to demonstrate that microbiota in both foregut and hindgut significantly changed after 3 or 14 days of antibiotics exposure, and the changes persisted over long period of time (>18 days) after withdrawing the antibiotics. We further observed a faster restoration of microbiota in both foregut and hindgut of MT group than CON group, microbiota in foregut was mainly benefited from microbiota transplantation by restoring the alpha-diversity as well as within-group similarity, while microbiota in hindgut was primarily benefited from microbiota transplantation by reestablishing the co-occurrence network (nodes number, edges number, density, modularity as well as closeness centrality). These results together expanded our understanding of restoration of the biased microbiota by antibiotics, and may also be instructive to deal with the delayed microbiota restoration at least in cows.
登录
查看更多内容
DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
6.7
作者：
Caballero S;Carter R;Ke X;Sušac B;Leiner IM;Kim GJ;Miller L;Ling L;Manova K;Pamer EG
通讯作者：
Pamer EG
影响因子：
5.2
作者：
Ji SK;Yan H;Jiang T;Guo CY;Liu JJ;Dong SZ;Yang KL;Wang YJ;Cao ZJ;Li SL
通讯作者：
Li SL
影响因子：
11
作者：
Fuentes, Susana;van Nood, Els;de Vos, Willem M.
通讯作者：
de Vos, Willem M.
影响因子：
3.7
作者：
Gu S;Cheung HH;Lee TL;Lu G;Poon WS;Chan WY
通讯作者：
Chan WY