Shigella flexneri effectors OspE1 and OspE2 mediate induced adherence to the colonic epithelium following bile salts exposure.
Shigella flexneri effectors OspE1 and OspE2 mediate induced adherence to the colonic epithelium following bile salts exposure.
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DOI：
10.1111/j.1365-2958.2012.08092.x
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发表时间：
2012-07
影响因子：
3.6
通讯作者：
Nataro JP
中科院分区：
文献类型：
--
作者：
Faherty CS;Redman JC;Rasko DA;Barry EM;Nataro JP
福氏志贺氏菌是一种侵入结肠上皮的革兰氏阴性病原体。虽然入侵已被彻底研究，但尚不清楚志贺氏菌如何首先附着在上皮上。先前的文献表明志贺氏菌利用由环境信号诱导的粘附素，包括在入侵小肠之前遇到的胆汁盐。我们假设胆汁会诱导粘附因子以促进与结肠上皮细胞的附着。为了检验我们的假设，福氏链球菌菌株 2457T 在含有胆汁盐的培养基中进行传代培养，并测量细菌粘附到极化 T84 上皮细胞顶端表面的能力。我们观察到粘附性显着增加，而毒力质粒治愈菌株和 III 型分泌系统突变体则没有这种情况。微阵列表达分析表明，ospE1/ospE2 基因在胆汁存在下被诱导，并且胆汁诱导的粘附在 ΔospE1/ΔospE2 突变体中消失。进一步的研究表明，暴露于胆汁盐后，OspE1/OspE2 蛋白定位于细菌外膜。所提供的数据首次证明 OspE1/OspE2 蛋白促进对肠上皮的初始粘附。志贺氏菌附着到结肠上皮所需的粘附素可以作为疫苗开发的理想靶标。
Shigella flexneri is a Gram-negative pathogen that invades the colonic epithelium. While invasion has been thoroughly investigated, it is unknown how Shigella first attaches to the epithelium. Previous literature suggests that Shigella utilizes adhesins that are induced by environmental signals, including bile salts, encountered in the small intestine prior to invasion. We hypothesized that bile would induce adherence factors to facilitate attachment to colonic epithelial cells. To test our hypothesis, S. flexneri strain 2457T was subcultured in media containing bile salts, and the ability of the bacteria to adhere to the apical surface of polarized T84 epithelial cells was measured. We observed a significant increase in adherence, which was absent in a virulence plasmid-cured strain and a type-III secretion system mutant. Microarray expression analysis indicated that the ospE1/ospE2 genes were induced in the presence of bile, and bile-induced adherence was lost in a ΔospE1/ΔospE2 mutant. Further studies demonstrated that the OspE1/OspE2 proteins were localized to the bacterial outer membrane following exposure to bile salts. The data presented are the first demonstration that the OspE1/OspE2 proteins promote initial adherence to the intestinal epithelium. The adhesins required for Shigella attachment to the colonic epithelium may serve as ideal targets for vaccine development.
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影响因子：
3.1
作者：
POPE, LM;REED, KE;PAYNE, SM
通讯作者：
PAYNE, SM
影响因子：
15.9
作者：
Kline KA;Dodson KW;Caparon MG;Hultgren SJ
通讯作者：
Hultgren SJ
影响因子：
3.1
作者：
Bahrani, FK;Sansonetti, PJ;Parsot, C
通讯作者：
Parsot, C
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
5.8
作者：
Hubbell, E;Liu, WM;Mei, R
通讯作者：
Mei, R