Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics.
Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics.
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DOI：
10.1155/2017/9306564
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发表时间：
2017
影响因子：
--
通讯作者：
Krieger MA
中科院分区：
文献类型：
--
作者：
Ali N;Rampazzo RCP;Costa ADT;Krieger MA
核酸提取(NAE)作为许多下游应用的首要步骤，在分子生物学中起着至关重要的作用。对最初的1869年方法进行了许多修改。现代工艺分为化学工艺或机械工艺，每种工艺都有影响其使用的特性，特别是在医疗诊断(POC-Dx)中。POC-Dx是一种新的方法，旨在用微量分析系统取代复杂的分析机器，能够在患者附近、护理地点或需要的地点使用。尽管已经做出了显著的努力，但简单有效的提取方法仍然是POC-Dx广泛使用的主要挑战。在这篇综述中，我们剖析了每种最常见的NAE方法的工作原理，概述了它们的优缺点，以及它们在POC-Dx系统中的集成潜力。目前，建立一个可以普遍适用于PoC-Dx的程序似乎很困难，如果不是不可能的话。我们还讨论了NAE化学品对用于批量生产POC-Dx系统的主要塑料聚合物的影响。我们在综述结束时讨论了应该指导对可集成到POC-Dx系统中的高效提取方法的追求的限制和挑战。
Nucleic acid extraction (NAE) plays a vital role in molecular biology as the primary step for many downstream applications. Many modifications have been introduced to the original 1869 method. Modern processes are categorized into chemical or mechanical, each with peculiarities that influence their use, especially in point-of-care diagnostics (POC-Dx). POC-Dx is a new approach aiming to replace sophisticated analytical machinery with microanalytical systems, able to be used near the patient, at the point of care or point of need. Although notable efforts have been made, a simple and effective extraction method is still a major challenge for widespread use of POC-Dx. In this review, we dissected the working principle of each of the most common NAE methods, overviewing their advantages and disadvantages, as well their potential for integration in POC-Dx systems. At present, it seems difficult, if not impossible, to establish a procedure which can be universally applied to POC-Dx. We also discuss the effects of the NAE chemicals upon the main plastic polymers used to mass produce POC-Dx systems. We end our review discussing the limitations and challenges that should guide the quest for an efficient extraction method that can be integrated in a POC-Dx system.
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影响因子：
2.9
作者：
CHOMCZYNSKI, P;SACCHI, N
通讯作者：
SACCHI, N
影响因子：
5
作者：
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通讯作者：
Berensmeier S
DOI：
10.1115/1.2798037
发表时间：
1999-02-01
影响因子：
1.7
作者：
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通讯作者：
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影响因子：
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