Magnetic particles for the separation and purification of nucleic acids.
Magnetic particles for the separation and purification of nucleic acids.
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DOI：
10.1007/s00253-006-0675-0
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发表时间：
2006-12
影响因子：
5
通讯作者：
Berensmeier S
中科院分区：
文献类型：
--
作者：
Berensmeier S
核酸分离是分子生物学中越来越重要的工具。在可以使用现代技术之前，核酸分离一直是基于若干提取和离心步骤的耗时和工作的过程，通常受到分离产物的低产率和低纯度的限制，并且不适合自动化和扩大规模。在过去的几年中，专门功能化的磁性颗粒被开发出来。与适当的缓冲系统一起，它们允许在从粗细胞提取物中提取后直接进行快速有效的纯化。避免了重复步骤。此外，新方法提供了整个过程的简单自动化和从更大的样品体积中分离核酸。本文综述了传统的核酸纯化方法和基于磁性粒子的核酸纯化方法。更详细地介绍了各种磁性颗粒的合成。列出了用于核酸分离的磁性颗粒的各种供应商以及提供用于各种不同样品材料的基于颗粒的试剂盒的供应商。此外，提及了用于磁性颗粒处理和液体处理的市售手动磁性分离器和自动化系统。
Nucleic acid separation is an increasingly important tool for molecular biology. Before modern technologies could be used, nucleic acid separation had been a time- and work-consuming process based on several extraction and centrifugation steps, often limited by small yields and low purities of the separation products, and not suited for automation and up-scaling. During the last few years, specifically functionalised magnetic particles were developed. Together with an appropriate buffer system, they allow for the quick and efficient purification directly after their extraction from crude cell extracts. Centrifugation steps were avoided. In addition, the new approach provided for an easy automation of the entire process and the isolation of nucleic acids from larger sample volumes. This review describes traditional methods and methods based on magnetic particles for nucleic acid purification. The synthesis of a variety of magnetic particles is presented in more detail. Various suppliers of magnetic particles for nucleic acid separation as well as suppliers offering particle-based kits for a variety of different sample materials are listed. Furthermore, commercially available manual magnetic separators and automated systems for magnetic particle handling and liquid handling are mentioned.
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