Specific deletions in the hepatitis B virus core open reading frame in patients with chronic active hepatitis B
Specific deletions in the hepatitis B virus core open reading frame in patients with chronic active hepatitis B
复制标题
慢性活动性乙型肝炎患者乙型肝炎病毒核心开放阅读框的特异性缺失
DOI：
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发表时间：
1993
影响因子：
12.7
通讯作者：
Roger Williams
中科院分区：
文献类型：
--
作者：
A. Ackrill;N. Naoumov;A. Eddleston;Roger Williams
应用聚合酶链式反应(PCR)和DNA测序技术检测慢性感染患者乙型肝炎病毒(HBVDNA)前C区/C区开放阅读框中的基因组变异。扩增产物的凝胶电泳显示，除了全长产物外，还存在核心基因的缩短形式。这些缩写形式仅见于HBeAg血清阳性的慢性活动性肝炎(CAH)患者，而不见于慢性迁延性肝炎(CPH)或HBeAg阴性的CAH患者。克隆和DNA测序显示，核心基因内存在许多重叠的缺失，大多数是框内缺失，聚集在核心基因产物的aa81-114内。这些缺失在不同种族和地理背景的CAH患者中被发现，而对表面和×开放阅读框的PCR分析显示，这些缺失没有缩短的形式，表明这些患者中存在针对核心基因的缺失。由于核心基因的产物--乙肝病毒核心抗原--被认为是T细胞介导的肝损伤的主要靶点，因此携带缺失的核心基因的产物可能会改变免疫识别，并可能在炎症性肝损伤的进展中起重要作用。
The polymerase chain reaction (PCR) and DNA sequencing were used to examine genomic variation in the pre‐core/core open reading frame of the hepatitis B virus (HBV) in chronically infected patients. Gel electrophoresis of amplification products showed the presence of shortened forms of the core gene in addition to the full length product. These shortened forms were seen only in patients with chronic active hepatitis (CAH) seropositive for HBeAg and not in patients with chronic persistent hepatitis (CPH) or HBeAg minus CAH. Cloning and DNA sequencing revealed the presence of a number of overlapping deletions within the core gene, the majority being in‐frame, which were clustered within aa 81‐114 of the core gene product. These deletions were found in patients with CAH from different racial and geographical backgrounds, whereas PCR analysis of the surface and ×open reading frames showed no shortened forms suggesting deletions to be specific to the core gene in these patients. Because the product of the core gene‐the HBV core antigen–is believed to be the major target for T‐cell‐mediated liver damage, it seems likely that the products of core genes carrying deletions will alter immune recognition and may be of importance in the progression of inflammatory liver damage.
DOI：
10.1073/pnas.83.6.1578
发表时间：
1986-03-01
影响因子：
11.1
作者：
OU, JH;LAUB, O;RUTTER, WJ
通讯作者：
RUTTER, WJ
DOI：
10.1073/pnas.88.23.10445
发表时间：
1991-12-01
影响因子：
11.1
作者：
BERTOLETTI, A;FERRARI, C;CHISARI, FV
通讯作者：
CHISARI, FV
克隆的肝内乙型肝炎病毒核蛋白特异性辅助 T 细胞系的功能特征。
DOI：
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发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Ferrari,C;Mondelli,MU;Penna,A;Fiaccadori,F;Chisari,FV
通讯作者：
Chisari,FV