Effect of different sintering methods on bioactivity and release of proteins from PLGA microspheres.
Effect of different sintering methods on bioactivity and release of proteins from PLGA microspheres.
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DOI：
10.1016/j.msec.2013.06.026
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发表时间：
2013-10
期刊：
影响因子：
--
通讯作者：
Detamore MS
中科院分区：
文献类型：
--
作者：
Dormer NH;Gupta V;Scurto AM;Berkland CJ;Detamore MS
Macromolecule release from poly(d,l-lactide-co-glycolide) (PLGA) microspheres has been well-characterized, and is a popular approach for delivering bioactive signals from tissue-engineered scaffolds. However, the effect of some processing solvents, sterilization, and mineral incorporation (when used in concert) on long-term release and bioactivity has seldom been addressed. Understanding these effects is of significant importance for microsphere-based scaffolds, given that these scaffolds are becoming increasingly more popular, yet growth factor activity following sintering and/or sterilization is heretofore unknown. The current study evaluated the 6-week release of transforming growth factor (TGF)-β3 and bone morphogenetic protein (BMP)-2 from PLGA and PLGA/hydroxyapatite (HAp) microspheres following exposure to ethanol (EtOH), dense phase carbon dioxide (CO2), or ethylene oxide (EtO). EtO was chosen based on its common use in scaffold sterilization, whereas EtOH and CO2 were chosen given their importance in sintering microspheres together to create scaffolds. Release supernatants were then used in an accelerated cell stimulation study with human bone marrow stromal cells (hBMSCs) with monitoring of gene expression for major chondrogenic and osteogenic markers. Results indicated that in microspheres without HAp, EtOH exposure led to the greatest amount of delivery, whilst those treated with CO2 delivered the least growth factor. In contrast, formulations with HAp released almost half as much protein, regardless of EtOH or CO2 exposure. Notably, EtO exposure was not found to significantly affect the amount of protein released. Cell stimulation studies demonstrated that eluted protein samples performed similarly to positive controls in PLGA-only formulations, and ambiguously in PLGA/HAp composites. In conclusion, the use of EtOH, subcritical CO2, and EtO in microsphere-based scaffolds may have only slight adverse effects, and possibly even desirable effects in some cases, on protein availability and bioactivity.
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影响因子：
3.8
作者：
Dormer, Nathan H.;Berkland, Cory J.;Detamore, Michael S.
通讯作者：
Detamore, Michael S.
DOI：
10.1016/j.jconrel.2008.10.019
发表时间：
2009-02-20
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Jay SM;Saltzman WM
通讯作者：
Saltzman WM
DOI：
10.1007/s10856-007-3050-5
发表时间：
2008-03-01
影响因子：
3.7
作者：
Fei, Zhengqi;Hu, Yunyu;Song, Hongxun
通讯作者：
Song, Hongxun
影响因子：
4.1
作者：
Ahn, Ji-Hyun;Lee, Tae-Hyeong;Im, Gun-Il
通讯作者：
Im, Gun-Il
影响因子：
10.8
作者：
Aubert-Pouëssel, A;Venier-Julienne, MC;Benoit, JP
通讯作者：
Benoit, JP