Caveolae preservation in the characterization of human neuromuscular disease
Caveolae preservation in the characterization of human neuromuscular disease
复制标题
人类神经肌肉疾病特征中的小窝保存
作者：
Robert E. Lee;Ann C. Poulos;R. F. Mayer;J. Rash
我们研究了通过灌流和常规浸泡固定方法制备的大鼠肌纤维的冷冻断裂复制品和常规薄片图像，以及通过类似的浸泡固定方法制备的正常和营养不良的人肌纤维的肌纤维。在大鼠和人的肌纤维中，小凹的大小和分布根据(1)戊二醛暴露的方法，(2)从接触固定剂的表面到肌纤维的深度，以及(3)如果手术切开，到肌纤维切断端的距离，被发现有很大的不同。传统的浸泡固定会导致肌膜凹陷不可避免但可预见的改变。这些可复制的固定技术使凹陷作为人类神经肌肉疾病特征的可靠标志物的使用变得非常复杂。
We have examined freeze‐fracture replicas and conventional thinsection images of rat myofibers prepared by perfusion and by conventional immersion fixation protocols, and myofibers of normal and dystrophic human myofibers prepared by similar immersion fixation methods. In both rat and human myofibers, the size and distribution of caveolae was found to differ substantially according to (1) the method of glutaraldehyde exposure, (2) the depth of the myofiber from the surface exposed to the fixative, and (3) if surgically bisected, the distance from the cut end of the myofiber. Conventional immersion fixation resulted in unavoidable but predictable alterations in sarcolemmal caveolae. These reproducible artifacts of fixation technique substantially complicate the use of caveolae as reliable markers for the characterization of human neuromuscular disease.
DOI：
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发表时间：
1981-08
期刊：
The American journal of pathology
影响因子：
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作者：
E. Bonilla;K. Fischbeck;D. Schotland
通讯作者：
E. Bonilla;K. Fischbeck;D. Schotland