Porphyromonas gingivalis diffusible signaling molecules enhance Fusobacterium nucleatum biofilm formation via gene expression modulation.
Porphyromonas gingivalis diffusible signaling molecules enhance Fusobacterium nucleatum biofilm formation via gene expression modulation.
复制标题
牙龈卟啉单胞菌可扩散的信号分子通过基因表达调控促进核梭杆菌生物膜的形成。
DOI：
10.1080/20002297.2023.2165001
复制
发表时间：
2023
影响因子：
4.5
通讯作者：
中科院分区：
文献类型：
--
作者：
Periodontitis is caused by a dysbiotic shift in the dental plaque microbiome. Fusobacterium nucleatum is involved in the colonization of Porphyromonas gingivalis, which plays a key role in dysbiosis, via coaggregation and synergy with this microorganism. We investigated the effect of diffusible signaling molecules from P. gingivalis ATCC 33277 on F. nucleatum TDC 100 to elucidate the synergistic mechanisms involved in dysbiosis. The two species were cocultured separated with an 0.4-µm membrane in tryptic soy broth, and F. nucleatum gene expression profiles in coculture with P. gingivalis were compared with those in monoculture. RNA sequencing revealed 139 genes differentially expressed between the coculture and monoculture. The expression of 52 genes was upregulated, including the coaggregation ligand-coding gene. Eighty-seven genes were downregulated. Gene Ontology analysis indicated enrichment for the glycogen synthesis pathway and a decrease in de novo synthesis of purine and pyrimidine. These results indicate that diffusible signaling molecules from P. gingivalis induce metabolic changes in F. nucleatum, including an increase in polysaccharide synthesis and reduction in de novo synthesis of purine and pyrimidine. The metabolic changes may accelerate biofilm formation by F. nucleatum with P. gingivalis. Further, the alterations may represent potential therapeutic targets for preventing dysbiosis.
登录
查看更多内容
影响因子：
5.4
作者：
Han YW
通讯作者：
Han YW
影响因子：
3.7
作者：
Ishihara K;Wawrzonek K;Shaw LN;Inagaki S;Miyamoto M;Potempa J
通讯作者：
Potempa J
Keystone-Pathogen假设。
DOI：
10.1038/nrmicro2873
发表时间：
2012-10
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
2.8
作者：
Genevaux, P;Bauda, P;Oudega, B
通讯作者：
Oudega, B
影响因子：
3.7
作者：
Ma J;Kageyama S;Takeshita T;Shibata Y;Furuta M;Asakawa M;Yamashita Y
通讯作者：
Yamashita Y