Intraspecific variability of cadmium tolerance and accumulation, and cadmium-induced cell wall modifications in the metal hyperaccumulator Arabidopsis halleri.
Intraspecific variability of cadmium tolerance and accumulation, and cadmium-induced cell wall modifications in the metal hyperaccumulator Arabidopsis halleri.
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DOI：
10.1093/jxb/erv144
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发表时间：
2015-06
影响因子：
6.9
通讯作者：
Verbruggen N
中科院分区：
文献类型：
--
作者：
Meyer CL;Juraniec M;Huguet S;Chaves-Rodriguez E;Salis P;Isaure MP;Goormaghtigh E;Verbruggen N
A.不同品种对Cd的耐性和积累量差异很大。halleri，并且耐受性、积累和土壤类型之间的关系不是直接的。镉诱导的细胞壁修饰表明不同的芽解毒机制。镉的耐受性和积累的某些分子机制已被确定在模式物种拟南芥，而种内变异的这些性状和地上部解毒的机制很少解决。研究了金属和非金属生绿僵菌对Cd的耐性和积累能力。在受控条件下测试来自不同遗传单位的Halleri种群。此外，拍摄细胞壁组成的变化进行了研究，使用傅里叶变换红外光谱。事实上，最近的工作A. halleri的研究表明，镉在细胞内和细胞壁/质外体中被螯合。全是A。halleri种群对Cd的耐受性最强，而金属性种群的耐受性最强。积累是高度可变的人群之间和人群内，和人口是非镉的。镉对敏感种A细胞壁组成的影响相似。lyrata和A. halleri个体;果胶/多糖含量的细胞壁似乎增加镉处理后。然而，镉诱导的变化更明显的耐受性较低的个人，导致的耐受性和修改的程度之间的相关性。本研究表明，镉的耐性和积累是一个高度变异的性状。halleri，表明适应在当地规模和参与各种分子机制。虽然在非metallicolous人口的细胞壁发生剧烈的修改，由于更高的镉毒性和/或镉固定在这个隔间，metallicolous人口的耐受性增加可能涉及其他机制，如液泡隔离。
A huge variability in Cd tolerance and accumulation exist within A. halleri, and the relationship between tolerance, accumulation, and edaphic type is not straightforward. Cd-induced cell wall modifications suggest various shoot detoxification mechanisms. Certain molecular mechanisms of Cd tolerance and accumulation have been identified in the model species Arabidopsis halleri, while intraspecific variability of these traits and the mechanisms of shoot detoxification were little addressed. The Cd tolerance and accumulation of metallicolous and non-metallicolous A. halleri populations from different genetic units were tested in controlled conditions. In addition, changes in shoot cell wall composition were investigated using Fourier transform infrared spectroscopy. Indeed, recent works on A. halleri suggest Cd sequestration both inside cells and in the cell wall/apoplast. All A. halleri populations tested were hypertolerant to Cd, and the metallicolous populations were on average the most tolerant. Accumulation was highly variable between and within populations, and populations that were non-accumulators of Cd were identified. The effect of Cd on the cell wall composition was quite similar in the sensitive species A. lyrata and in A. halleri individuals; the pectin/polysaccharide content of cell walls seems to increase after Cd treatment. Nevertheless, the changes induced by Cd were more pronounced in the less tolerant individuals, leading to a correlation between the level of tolerance and the extent of modifications. This work demonstrated that Cd tolerance and accumulation are highly variable traits in A. halleri, suggesting adaptation at the local scale and involvement of various molecular mechanisms. While in non-metallicolous populations drastic modifications of the cell wall occur due to higher Cd toxicity and/or Cd immobilization in this compartment, the increased tolerance of metallicolous populations probably involves other mechanisms such as vacuolar sequestration.
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影响因子：
9.4
作者：
Frerot, Helene;Faucon, Michel-Pierre;Saumitou-Laprade, Pierre
通讯作者：
Saumitou-Laprade, Pierre
影响因子：
8.9
作者：
Dahmani-Muller, H;van Oort, F;Balabane, M
通讯作者：
Balabane, M
影响因子：
9.4
作者：
Assunçao, AGL;Bookum, WM;Ernst, WHO
通讯作者：
Ernst, WHO
DOI：
10.1199/tab.0001
发表时间：
2002-01-01
期刊：
The arabidopsis book
影响因子：
--
作者：
Al-Shehbaz, Ihsan A;O'Kane, Steve L Jr
通讯作者：
O'Kane, Steve L Jr
影响因子：
6.9
作者：
Craciun, Adrian R.;Meyer, Claire-Lise;Verbruggen, Nathalie
通讯作者：
Verbruggen, Nathalie