Characterization of the radioresponse of human apical papilla-derived cells.
Characterization of the radioresponse of human apical papilla-derived cells.
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DOI：
10.1186/scrt43
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发表时间：
2011-01-20
影响因子：
7.5
通讯作者：
Miura M
中科院分区：
文献类型：
--
作者：
Abe S;Hamada K;Yamaguchi S;Amagasa T;Miura M
本研究旨在对比根尖乳头来源细胞（APDCs）中的干/祖细胞与普通APDCs的放射生物学特性。
APDCs从新拔除的根尖未发育成熟的人类第三磨牙中分离获得。利用神经球培养技术，APDCs形成了多能球，人们认为其中富含干/祖细胞。经γ射线照射后，对乳头球形成细胞（PSFCs）和普通APDCs进行放射敏感性及硬组织形成试验。
与普通APDCs相比，PSFCs表现出放射抗性表型，且具有更强的DNA双链断裂修复能力。照射诱导两种细胞类型均出现显著的衰老样表型。两种细胞在接受8戈瑞（Gy）照射后，均未出现明显的凋亡变化。在接受4 Gy照射后，PSFCs的体内硬组织形成能力显著下降，而普通APDCs则未出现这种情况。
我们首次证明，源自APDCs的干/祖细胞表现出放射抗性表型；然而，经照射后，其体内硬组织形成能力显著降低，而普通APDCs则无此现象。
The purpose of this study was to characterize the radiobiological properties of stem/progenitor cells derived from apical papilla-derived cells (APDCs) compared to bulk APDCs. APDCs were isolated from freshly extracted human third molars with immature apices. Multipotent spheres, which are thought to contain an enriched population of stem/progenitor cells, were formed from the APDCs, using a neurosphere culture technique. After γ-irradiation, papillary sphere-forming cells (PSFCs) and bulk APDCs were subjected to radiosensitivity and hard tissue-forming assays. Compared to bulk APDCs, the PSFCs exhibited a radioresistant phenotype and a higher capacity for DNA double strand break repair. Irradiation induced a significant increase in a senescence-like phenotype in both cell types. Neither type of cells exhibited a significant induction of apoptotic changes after 8 Gy of irradiation. Ability to form hard tissue in vivo was significantly decreased in PSFCs, but not in APDCs following 4 Gy of irradiation. We demonstrated for the first time that stem/progenitor cells derived from APDCs exhibit a radioresistant phenotype; however, the hard tissue forming ability in vivo, but not bulk APDCs, was significantly reduced after irradiation.
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影响因子：
3.7
作者：
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发表时间：
1995-09-26
影响因子：
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DOI：
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发表时间：
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影响因子：
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作者：
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Ohgushi, H