Pomegranate fruit extract inhibits UVB-induced inflammation and proliferation by modulating NF-κB and MAPK signaling pathways in mouse skin.
Pomegranate fruit extract inhibits UVB-induced inflammation and proliferation by modulating NF-κB and MAPK signaling pathways in mouse skin.
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DOI：
10.1111/j.1751-1097.2011.01063.x
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发表时间：
2012-09
影响因子：
3.3
通讯作者：
Afaq F
中科院分区：
文献类型：
--
作者：
Khan N;Syed DN;Pal HC;Mukhtar H;Afaq F
人们对能够保护皮肤免受太阳紫外线B(UVB)辐射不利影响的天然制剂非常感兴趣。石榴(石榴L)果实具有很强的抗氧化、抗炎和抗增殖作用。最近，我们已经证明，口服石榴果提取物(PFE)给小鼠提供了实质性的保护，使其免受单一UVB辐射的不利影响，这是通过调节光致癌的早期生物标志物来实现的。本研究旨在探讨PFE(0.2%，w/v)对SKH-1无毛小鼠皮肤经多次UVB(180mJ/cm2；隔日照射，共7次照射)后的光化学预防作用。SKH-1小鼠口服PFE可抑制UVB诱导的表皮增生、白细胞渗入、蛋白质氧化和脂质过氧化。免疫印迹分析表明，口服PFE可抑制中波紫外线诱导的(1)核转位和核蛋白κB/p65的磷酸化，(2)IκBα的磷酸化和降解，(3)IKKα/IKKβ的激活，(4)MAPK蛋白和c-jun的磷酸化。PFE还可抑制UVB诱导的小鼠皮肤中(I)COX-2和iNOS，(Ii)增殖细胞核抗原和细胞周期蛋白D1，(Iii)基质金属蛋白酶-2，-3和-9的蛋白表达。综上所述，这些数据表明，PFE的消耗通过调节UVB诱导的信号通路为小鼠皮肤提供了保护，使其免受UVB辐射的不利影响。
There is considerable interest in the identification of natural agents capable of affording protection to skin from the adverse effects of solar ultraviolet B (UVB) radiation. Pomegranate (Punica granatum L) fruit possess strong anti-oxidant, anti-inflammatory and anti-proliferative properties. Recently, we have shown that oral feeding of pomegranate fruit extract (PFE) to mice afforded substantial protection from the adverse effects of single UVB radiation via modulation in early biomarkers of photocarcinogenesis. This study was designed to investigate the photochemopreventive effects of PFE (0.2%, w/v) after multiple UVB irradiations (180 mJ/cm2; on alternative day; for a total of seven treatments) to the skin of SKH-1 hairless mice. Oral feeding of PFE to SKH-1 mice inhibited UVB-induced epidermal hyperplasia, infiltration of leukocytes, protein oxidation and lipid peroxidation. Immunoblot analysis demonstrated that oral feeding of PFE to mice inhibited UVB-induced (i) nuclear translocation and phosphorylation of NF-κB/p65, (ii) phosphorylation and degradation of IκBα, (iii) activation of IKKα/IKKβ, and (iv) phosphorylation of MAPK proteins and c-Jun. PFE consumption also inhibited UVB-induced protein expression of (i) COX-2 and iNOS, (ii) PCNA and cyclin D1, and (iii) matrix metalloproteinases-2,-3 and -9 in mouse skin. Taken together, these data show that PFE consumption afforded protection to mouse skin against the adverse effects of UVB radiation by modulating UVB-induced signaling pathways.
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影响因子：
6.4
作者：
Afaq, F;Saleem, M;Mukhtar, H
通讯作者：
Mukhtar, H
影响因子：
3.9
作者：
Afaq F
通讯作者：
Afaq F
影响因子：
8
作者：
Afaq, F;Ahmad, N;Mukhtar, H
通讯作者：
Mukhtar, H
影响因子：
10.5
作者：
Coussens, LM;Raymond, WW;Hanahan, D
通讯作者：
Hanahan, D
影响因子：
3.8
作者：
Katiyar, SK;Afaq, F;Mukhtar, H
通讯作者：
Mukhtar, H