C-X-C Chemokines Influence Intraocular Inflammation During Bacillus Endophthalmitis.
C-X-C Chemokines Influence Intraocular Inflammation During Bacillus Endophthalmitis.
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DOI：
10.1167/iovs.62.14.14
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发表时间：
2021-11-01
影响因子：
4.4
通讯作者：
Callegan MC
中科院分区：
文献类型：
--
作者：
Mursalin MH;Coburn PS;Miller FC;Livingston ET;Astley R;Callegan MC
本研究的目的是探索C-X-C趋化因子CXCL 2和CXCL 10作为芽孢杆菌眼内炎的潜在抗炎靶点。 在C57 BL/6 J、CXCL 2 −/−和CXCL 10 −/−小鼠中诱导了芽孢杆菌眼内炎。在感染后的特定时间，分析眼睛的芽孢杆菌，视网膜功能和炎症。在B中，玻璃体内抗CXCL 2和抗CXCL 10联合或不联合加替沙星的疗效。还使用相同的技术评估蜡样眼内炎。尽管C57 BL/6 J、CXCL 2 −/−和CXCL 10 −/−小鼠眼睛中的芽孢杆菌生长相似，但与C57 BL/6 J小鼠相比，CXCL 2 −/−和CXCL 10 −/−小鼠眼睛中的视网膜功能保留更大。与C57 BL/6 J小鼠的眼睛相比，CXCL 2 −/−和CXCL 10 −/−小鼠眼睛中的神经元迁移减少的程度更大。感染CXCL 2 −/−和CXCL 10 −/−的小鼠眼睛的炎症明显少于C57 BL/6 J的眼睛。CXCL 2 −/−小鼠受感染眼睛的视网膜结构比CXCL 10 −/−眼睛保存的时间更长。与未治疗的眼睛相比，在用抗CXCL 2和抗CXCL 10加或不加加替沙星治疗的眼睛中，炎症较少，视网膜功能显著保留。对于芽孢杆菌眼内炎，小鼠中CXCL 2或CXCL 10的缺乏导致视网膜功能保留和炎症减少。不存在CXCL 2导致比不存在CXCL 10更好的临床结果。在B期间使用抗CXCL 2和抗CXCL 10限制了炎症。蜡样眼内炎这些结果强调了CXCL 2和CXCL 10作为抗炎治疗潜在靶点的实用性，可以与抗生素联合测试以改善杆菌性眼内炎的治疗。
The purpose of this study was to explore the C-X-C chemokines CXCL2 and CXCL10 as potential anti-inflammatory targets for Bacillus endophthalmitis. Bacillus endophthalmitis was induced in C57BL/6J, CXCL2−/−, and CXCL10−/− mice. At specific times postinfection, eyes were analyzed for Bacillus, retinal function, and inflammation. The efficacies of intravitreal anti-CXCL2 and anti-CXCL10 with or without gatifloxacin in B. cereus endophthalmitis were also assessed using the same techniques. Despite similar Bacillus growth in eyes of C57BL/6J, CXCL2−/−, and CXCL10−/− mice, retinal function retention was greater in eyes of CXCL2−/− and CXCL10−/− mice compared to that of C57BL/6J mice. Neutrophil migration into eyes of CXCL2−/− and CXCL10−/− mice was reduced to a greater degree compared to that of eyes of C57BL/6J mice. Infected CXCL2−/− and CXCL10−/− mouse eyes had significantly less inflammation compared to that of C57BL/6J eyes. Retinal structures in infected eyes of CXCL2−/− mice were preserved for a longer time than in CXCL10−/− eyes. Compared to untreated eyes, there was less inflammation and significant retention of retinal function in eyes treated with anti-CXCL2 and anti-CXCL10 with or without gatifloxacin. For Bacillus endophthalmitis, the absence of CXCL2 or CXCL10 in mice resulted in retained retinal function and less inflammation. The absence of CXCL2 led to a better clinical outcome than the absence of CXCL10. The use of anti-CXCL2 and anti-CXCL10 limited inflammation during B. cereus endophthalmitis. These results highlight the utility of CXCL2 and CXCL10 as potential targets for anti-inflammatory therapy that can be tested in conjunction with antibiotics for improving treating Bacillus endophthalmitis.
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影响因子：
29.7
作者：
Brubaker SW;Bonham KS;Zanoni I;Kagan JC
通讯作者：
Kagan JC
影响因子：
3.1
作者：
Callegan, MC;Booth, MC;Gilmore, MS
通讯作者：
Gilmore, MS
DOI：
10.1099/mic.0.001057
发表时间：
2021-05
期刊：
Microbiology (Reading, England)
影响因子：
--
作者：
Coburn PS;Miller FC;Enty MA;Land C;LaGrow AL;Mursalin MH;Callegan MC
通讯作者：
Callegan MC
创伤后内嗜性：发病率和危险因素。
DOI：
10.5539/gjhs.v6n6p68
发表时间：
2014-06-30
期刊：
Global journal of health science
影响因子：
--
作者：
Dehghani AR;Rezaei L;Salam H;Mohammadi Z;Mahboubi M
通讯作者：
Mahboubi M
影响因子：
3.1
作者：
Callegan, MC;Cochran, DC;Lereclus, D
通讯作者：
Lereclus, D