Impact of menthol on nicotine intake and preference in mice: Concentration, sex, and age differences.
Impact of menthol on nicotine intake and preference in mice: Concentration, sex, and age differences.
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薄荷脑对小鼠尼古丁摄取和偏好的影响：浓度、性别和年龄差异。
DOI：
10.1016/j.neuropharm.2020.108274
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发表时间：
2020-11-15
影响因子：
4.7
通讯作者：
Damaj MI
中科院分区：
文献类型：
--
作者：
Bagdas D;Jackson A;Carper M;Chen RY;Akinola LS;Damaj MI
已经证明，薄荷有助于烟草产品对人类的吸引力。然而，诸如性别、年龄和薄荷醇浓度等因素在薄荷醇和尼古丁之间的相互作用中仍然不清楚。为了了解这些因素，我们利用小鼠模型来确定薄荷醇对口服尼古丁消耗的影响。在青少年和成年雌性和雄性C57 BL/6 J小鼠中，使用两瓶选择范例，在有或没有口服尼古丁的情况下测试了一系列薄荷醇浓度（口服和全身）。此外，使用转基因小鼠来研究α7烟碱乙酰胆碱受体（nAChRs）在薄荷醇作用中的作用。尼古丁溶液中添加薄荷醇以性别和薄荷醇浓度依赖性方式增加C57 BL/6 J小鼠的口服尼古丁消耗量。在较低的薄荷醇浓度下，雌性小鼠表现出尼古丁消耗的增强，而雄性小鼠在较高的薄荷醇浓度下表现出类似的行为。仅在薄荷醇浓度为60 μg/ml时，单独饮用薄荷醇在性别上存在显著差异，其中雌性小鼠的薄荷醇摄入量高于雄性小鼠。口服和全身（腹膜内）给予的薄荷脑增加了口服尼古丁消耗。与成年小鼠相比，青春期雌性小鼠在薄荷脑浓度较低的情况下尼古丁摄入量较高。虽然α7 nAChR野生型小鼠消耗的薄荷醇尼古丁溶液多于仅尼古丁溶液，但在KO小鼠中消除了该效应。薄荷醇的效应具有浓度、性别、年龄和α7 nAChR依赖性。口服和腹膜内薄荷醇增加尼古丁摄入，表明感觉、外周和/或中枢机制参与薄荷醇对口服尼古丁消耗的影响。
Menthol has been shown to contribute to the appeal of tobacco products in humans. However, factors such as sex, age and menthol concentration remain unclear in the interaction between menthol and nicotine. To understand these factors, we utilized a mouse model to determine the impact of menthol on oral nicotine consumption. A range of menthol concentrations (oral and systemic) were tested with or without oral nicotine using the two-bottle choice paradigm in adolescent and adult female and male C57BL/6J mice. Moreover, genetically modified mice were used to investigate the role of α7 nicotinic acetylcholine receptors (nAChRs) on the effects of menthol. Menthol addition to nicotine solution increased oral nicotine consumption in C57BL/6J mice in a sex- and menthol concentration-dependent manner. At lower menthol concentrations, female mice demonstrated an enhancement of nicotine consumption and male mice showed a similar behavior at higher menthol concentrations. Menthol drinking alone was only significantly different by sex at 60 μg/ml menthol concentration where female mice had higher menthol intake than males. Menthol administered both orally and systemically (intraperitoneal) increased oral nicotine consumption. Adolescent female mice had a higher nicotine intake at lower menthol concentrations compared to their adult counterparts. While α7 nAChR wild type mice consumed more mentholated nicotine solution than nicotine only solution, this effect was abolished in KO mice. Effects of menthol are concentration-, sex-, age-, and α7 nAChR-dependent. Oral and intraperitoneal menthol increases nicotine intake, suggesting that sensory, peripheral, and/or central mechanisms are involved in effects of menthol on oral nicotine consumption.
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影响因子：
4.7
作者：
Kong, Grace;Morean, Meghan E.;Krishnan-Sarin, Suchitra
通讯作者：
Krishnan-Sarin, Suchitra
影响因子：
3.7
作者：
Ashoor, Abrar;Nordman, Jacob C.;Oz, Murat
通讯作者：
Oz, Murat
影响因子：
5.1
作者：
Garten, S;Falkner, RV
通讯作者：
Falkner, RV
DOI：
10.1038/npp.2011.299
发表时间：
2012-04
期刊：
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.4
作者：
Henderson, Brandon J;Grant, Stephen;Lester, Henry A
通讯作者：
Lester, Henry A