RNA Sequencing Reveals Phenylpropanoid Biosynthesis Genes and Transcription Factors for Hevea brasiliensis Reaction Wood Formation.
RNA Sequencing Reveals Phenylpropanoid Biosynthesis Genes and Transcription Factors for Hevea brasiliensis Reaction Wood Formation.
复制标题
RNA测序揭示巴西橡胶树反应木材形成的苯丙类生物合成基因和转录因子。
DOI:
10.3389/fgene.2021.763841
复制
发表时间:
2021
影响因子:
3.7
通讯作者:
Tu Z
中科院分区:
文献类型:
--
作者:
Meng X;Wang Y;Li J;Jiao N;Zhang X;Zhang Y;Chen J;Tu Z
Given the importance of wood in many industrial applications, much research has focused on wood formation, especially lignin biosynthesis. However, the mechanisms governing the regulation of lignin biosynthesis in the rubber tree (Hevea brasiliensis) remain to be elucidated. Here, we gained insight into the mechanisms of rubber tree lignin biosynthesis using reaction wood (wood with abnormal tissue structure induced by gravity or artificial mechanical treatment) as an experimental model. We performed transcriptome analysis of rubber tree mature xylem from tension wood (TW), opposite wood (OW), and normal wood (NW) using RNA sequencing (RNA-seq). A total of 214, 1,280, and 32 differentially expressed genes (DEGs) were identified in TW vs. NW, OW vs. NW, and TW vs. OW, respectively. GO and KEGG enrichment analysis of DEGs from different comparison groups showed that zeatin biosynthesis, plant hormone signal transduction, phenylpropanoid biosynthesis, and plant–pathogen interaction pathways may play important roles in reaction wood formation. Sixteen transcripts involved in phenylpropanoid biosynthesis and 129 transcripts encoding transcription factors (TFs) were used to construct a TF–gene regulatory network for rubber tree lignin biosynthesis. Among them, MYB, C2H2, and NAC TFs could regulate all the DEGs involved in phenylpropanoid biosynthesis. Overall, this study identified candidate genes and TFs likely involved in phenylpropanoid biosynthesis and provides novel insights into the mechanisms regulating rubber tree lignin biosynthesis.
登录
查看更多内容
影响因子:
4.3
作者:
Herrero, Joaquin;Fernandez-Perez, Francisco;Miguel Zapata, Jose
通讯作者:
Miguel Zapata, Jose
影响因子:
4.4
作者:
Chen J;Chen B;Zhang D
通讯作者:
Zhang D
影响因子:
4.9
作者:
Li, Chaofeng;Wang, Xianqiang;Luo, Keming
通讯作者:
Luo, Keming
影响因子:
4.8
作者:
Han Xue-yuan;Mao Lin-chun;Luo Zi-sheng
通讯作者:
Luo Zi-sheng
影响因子:
4.4
作者:
Li X;Yang X;Wu HX
通讯作者:
Wu HX