Infrared linear dichroism reveals that A-, B-, and C-DNAs in films have bases highly inclined from perpendicular to the helix axis.
Infrared linear dichroism reveals that A-, B-, and C-DNAs in films have bases highly inclined from perpendicular to the helix axis.
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红外线性二色性表明薄膜中的 A-、B- 和 C-DNA 的碱基高度倾斜于垂直于螺旋轴的方向。
DOI：
10.1021/bi00193a021
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发表时间：
1994
期刊：
影响因子：
2.9
通讯作者：
JohnsonJr,WC
中科院分区：
文献类型：
--
作者：
Kang,H;JohnsonJr,WC
摘要：红外线性二色性已被用于研究聚[d（AC）]-聚[d（GT）]、聚[d（AG）]-聚[d（CT）]和天然DNA（来自大肠杆菌和小牛胸腺）膜中碱基的倾斜。所有研究的DNA在高相对湿度（> 94%）下呈现B型。聚-[d（AC）]>聚[d（GT）]，E.大肠杆菌DNA和小牛胸腺DNA在低（75%）相对湿度下呈A型，而poly [d（AG）]» poly [d（CT）]在低（66%）相对湿度下呈C型。红外线性二色性表明，DNA在膜中的碱基高度倾斜，从垂直于螺旋轴，即使是B-DNA。C-DNA与B-DNA具有几乎相同的倾斜度，而A-DNA的倾斜度略有增加。这些倾角证实了我们早期的紫外线线性二色性结果的DNA在溶液中的碱基的取向。红外线性二色性也被用来获得构象角的磷酸二酯骨架的几何形状的A-，B-和C-形式的DNA.The最近发展的技术分析的解决方案的生物聚合物的结构，如光谱，FTIR和NMR光谱，揭示了在水溶液中的核酸的结构细节，这类似于在活细胞的内部的真实的环境。但是，尽管在研究核酸在溶液状态和固体状态下的结构方面取得了进展，但DNA的分子结构仍然不确定。这主要是由于DNA的多态性，因为结构取决于序列（Bram &
Revised Manuscript Received May 5, 1994® abstract: Infrared linear dichroism has been employed to investigate the inclination of the bases in films of poly [d (AC)]-poly [d (GT)], poly [d (AG)]-poly [d (CT)], and natural DNAs (from Escherichia coli and calf thymus). All DNAs investigated assume the B-form at high (> 94%) relative humidity. Poly-[d (AC)]» poly [d (GT)], E. coli DNA, and calf thymus DNA assume the A-form at low (75%) relativehumidity, whereas poly [d (AG)]» poly [d (CT)] assumes the C-form at low (66%) relative humidity. Infrared linear dichroism demonstrates that the bases for DNA in films are highly inclined from perpendicular to the helix axis, even for B-DNA. C-DNA has almost same inclinations as in B-DNA, and the inclinations are slightly increased in A-DNA. These inclination angles confirm our earlier UV linear dichroism results for the orientation of the bases for DNA in solution. Infrared linear dichroism has also been used to obtain conformational angles for the phosphodiester backbone geometry of the A-, B-, and C-forms of DNA.The recent development of techniques for analyzing solution structures of biopolymers, such as optical spectroscopy, FTIR, and NMR spectroscopy, reveals the structural details of nucleic acids in aqueous solution, which resembles the real environment in the interior of living cells. But despite progress in studying nucleic acid structure in the solution state as well as in solid state, there is still uncertaintyabout the molecular structure of DNA. This is due mainly to the polymorphic nature of DNA, since the structure depends on the sequence (Bram &
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1-甲基胸腺嘧啶、9-甲基腺嘌呤及其1:1配合物单晶的红内吸收光谱
DOI：
10.1016/0584-8539(67)80022-9
发表时间：
1967
期刊：
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
影响因子：
--
作者：
Y. Kyōgoku;S. Higuchi;M. Tsuboi
通讯作者：
M. Tsuboi
通过 2D NMR、光谱模拟、分子建模和距离几何计算得出 GCCAAT 识别基序的溶液结构。
影响因子：
2.9
作者：
R. Nibedita;R. A. Kumar;A. Majumdar;R. Hosur;G. Govil;K. Majumder;Virander S. Chauhan
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Virander S. Chauhan
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影响因子：
5.6
作者：
B. Nordén;C. Elvingson;M. Kubista;B. Sjöberg;H. Ryberg;M. Ryberg;K. Mortensen;M. Takahashi
通讯作者：
M. Takahashi
DNA螺旋结构的起源及其序列依赖性。
影响因子：
2.9
作者：
Sarai,A;Mazur,J;Nussinov,R;Jernigan,RL
通讯作者：
Jernigan,RL
影响因子：
6.1
作者：
E. Charney
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