Development of a Microloading Platform for In Vitro Mechanotransduction Studies
Development of a Microloading Platform for In Vitro Mechanotransduction Studies
复制标题
用于体外机械传导研究的微加载平台的开发
DOI：
10.1007/978-3-319-06974-6_8
复制
发表时间：
2015
影响因子：
3.5
通讯作者：
M. Saunders
中科院分区：
文献类型：
--
作者：
S. L. York;Jonathan D. King;A. S. Pietros;B.;P. Sethu;M. Saunders
机械转导研究旨在了解细胞将机械刺激转化为细胞反应的过程。由于使用体内研究难以研究孤立因素的影响，因此使用体外研究，因为它们为实验提供了更精确的受控负载，并允许在各种表面上进行细胞培养。在这里，我们开发了一个微加载平台，在体外mechanotransduction研究，拉伸基板帐篷它与中央接触压板。该平台通过使用测力传感器和微致动器来工作，其特征在于将报告的位移与测量的位移进行比较。此外，为微加载平台设计了一个对齐块，以增加研究之间的再现性，并设计了一个细胞培养处理系统，以在实验前保持样品并减少预加载，从而仅允许进行受控加载的研究。还设计了一种聚二甲基硅氧烷（PDMS）支架用于细胞加载，并配有位置参考网格，用于观察单个细胞对应变的反应。该微负载平台的初步工作包括研究骨细胞样MLO-Y 4细胞和体外响应机械负载的活力变化。这些初步研究已经证明了诱导细胞死亡的能力，以应对微损伤。
Mechanotransduction studies aim to understand the process of cells converting mechanical stimuli into a cellular response. As it can be difficult to study the impact of isolated factors using in vivo studies, in vitro studies are used as they offer more precisely controlled loads for experiments and allow cell culture on a variety of surfaces. Here, we developed a microloading platform for in vitro mechanotransduction studies, stretching the substrate by tenting it with a centrally contacting platen. This platform works through the use of a load cell and microactuator, which was characterized by comparing the reported and measured displacements. In addition, an alignment block was designed for the microloading platform to increase reproducibility between studies, and a cell culture handling system was designed to hold samples before experimentation and reduce preloads, allowing the study of only the controlled loading. A polydimethylsiloxane (PDMS) scaffold was also designed for cell loading, complete with a positional reference grid for observing the response of individual cells to strain. Initial work with this microloading platform includes studying osteocyte-like MLO-Y4 cells and changes in viability in response to mechanical load in vitro. These initial studies have demonstrated the ability to induce cell death in response to microdamage.
影响因子：
5.5
作者：
Taylor, A. F.;Saunders, M. M.;Donahue, H. J.
通讯作者：
Donahue, H. J.
影响因子：
2.4
作者：
Bonivtch, Amber Rath;Bonewald, Lynda F.;Nicolella, Daniel P.
通讯作者：
Nicolella, Daniel P.