Chlamydia trachomatis Slc1 is a type III secretion chaperone that enhances the translocation of its invasion effector substrate TARP.
Chlamydia trachomatis Slc1 is a type III secretion chaperone that enhances the translocation of its invasion effector substrate TARP.
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DOI：
10.1111/j.1365-2958.2011.07802.x
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发表时间：
2011-10
影响因子：
3.6
通讯作者：
Carabeo RA
中科院分区：
文献类型：
--
作者：
Brinkworth AJ;Malcolm DS;Pedrosa AT;Roguska K;Shahbazian S;Graham JE;Hayward RD;Carabeo RA
细菌III型分泌系统（t3ss）在分泌能力状态下陪伴先导底物到输出装置，因此对效应器转运到靶细胞至关重要。沙眼衣原体是一种遗传上难以处理的专性细胞内病原体，利用T3SS效应物触发其进入哺乳动物细胞。唯一表征良好的T3SS效应物是TARP（易位肌动蛋白募集蛋白），但其伴侣是未知的。在这里，我们利用已知的结构特征来筛选沙眼衣原体基因组中编码的假定的III型分泌伴侣。利用细菌双杂交、共沉淀、交联和尺寸排除色谱，我们发现Slc1 （syce样伴侣1；CT043）特异性地与TARP （TARP1-200）的200个氨基酸残基n端区域相互作用。交联和凝胶过滤实验表明，Slc1在体外形成同型二聚体。分离的Slc1-TARP1-200配合物的生化分析符合2:1的伴侣效应化学计量学特征。此外，Slc1与沙眼衣原体的TARP共免疫沉淀。此外，Slc1的共表达特异性地增强了异源小肠结肠炎耶尔森菌T3SS对TARP的宿主细胞易位。综上所述，我们认为Slc1可能是沙眼衣原体T3SS效应物TARP的伴侣。
Bacterial type III secretion system (T3SSs) chaperones pilot substrates to the export apparatus in a secretion-competent state, and are consequently central to the translocation of effectors into target cells. Chlamydia trachomatis is a genetically intractable obligate intracellular pathogen that utilizes T3SS effectors to trigger its entry into mammalian cells. The only well-characterized T3SS effector is TARP (translocated actin recruitment protein), but its chaperone is unknown. Here we exploited a known structural signature to screen for putative type III secretion chaperones encoded within the C. trachomatis genome. Using bacterial two-hybrid, co-precipitation, cross-linking, and size exclusion chromatography we show that Slc1 (SycE-like chaperone 1; CT043) specifically interacts with a 200 amino acid residue N-terminal region of TARP (TARP1–200). Slc1 formed homodimers in vitro, as shown in crosslinking and gel filtration experiments. Biochemical analysis of an isolated Slc1-TARP1–200 complex was consistent with a characteristic 2:1 chaperone-effector stoichiometry. Furthermore, Slc1 was co-immunoprecipitated with TARP from C. trachomatis elementary bodies. Also, co-expression of Slc1 specifically enhanced host cell translocation of TARP by a heterologous Yersinia enterocolitica T3SS. Taken together, we propose Slc1 as a chaperone of the C. trachomatis T3SS effector TARP.
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影响因子：
3.6
作者：
Hower S;Wolf K;Fields KA
通讯作者：
Fields KA
DOI：
10.1073/pnas.0603044103
发表时间：
2006-10-17
影响因子：
11.1
作者：
Jewett, Travis J.;Fischer, Elizabeth R.;Hackstadt, Ted
通讯作者：
Hackstadt, Ted
无细胞沙眼衣原体原体诱导 III 型分泌。
影响因子：
3.8
作者：
Jamison, Wendy P.;Hackstadt, Ted
通讯作者：
Hackstadt, Ted
影响因子：
3.6
作者：
Lee, VT;Anderson, DM;Schneewind, O
通讯作者：
Schneewind, O
影响因子：
3.2
作者：
Fraser, GM;González-Pedrajo, B;Macnab, RM
通讯作者：
Macnab, RM