Coordinated miRNA/mRNA expression profiles for understanding breed-specific metabolic characters of liver between Erhualian and large white pigs.
Coordinated miRNA/mRNA expression profiles for understanding breed-specific metabolic characters of liver between Erhualian and large white pigs.
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DOI：
10.1371/journal.pone.0038716
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zhao R
中科院分区：
文献类型：
--
作者：
Li R;Sun Q;Jia Y;Cong R;Ni Y;Yang X;Jiang Z;Zhao R
MicroRNA (miRNA) 参与肝脏各种代谢过程的调节，但人们对 miRNA 与 mRNA 协调的品种特异性表达谱知之甚少。在这里，我们使用具有不同代谢特征的两个品种的雄性新生仔猪——大白猪（LW）和二花莲（EHL），利用微阵列描绘了mRNA的肝脏表达谱，并利用深度测序和微阵列描绘了miRNA的肝脏表达谱，并分析了整合的miRNA和mRNA表达与生理生化参数的功能相关性。 EHL出生时体重和肝脏重量明显较低，但血清总胆固醇（TCH）、高密度脂蛋白胆固醇（HDLC）和低密度脂蛋白胆固醇（LDLC）水平升高，肝脏胆固醇含量较高。在 EHL 仔猪中也观察到较高的血清皮质醇和较低的血清胰岛素和瘦素。与LW相比，EHL肝脏中鉴定出30个上调和18个下调的miRNA，以及298个上调和510个下调的mRNA（FDR<10%）。对一些差异表达 miRNA (DEM) 的 RT-PCR 验证进一步证实了高通量数据分析。使用目标预测算法，我们发现上调的 miRNA 和下调的 mRNA 之间存在显着相关性。此外，参与蛋白水解的差异表达基因（DEG）预计是由 DEM 介导的。这些发现提供了关于猪肝脏中miRNA和mRNA谱的新信息，这将有助于揭示猪品种特异性性状背后的分子机制，并可能为进一步研究猪肝脏中miRNA的生物学功能奠定基础。
MicroRNAs (miRNAs) are involved in the regulation of various metabolic processes in the liver, yet little is known on the breed-specific expression profiles of miRNAs in coordination with those of mRNAs. Here we used two breeds of male newborn piglets with distinct metabolic characteristics, Large White (LW) and Erhualian (EHL), to delineate the hepatic expression profiles of mRNA with microarray and miRNAs with both deep sequencing and microarray, and to analyze the functional relevance of integrated miRNA and mRNA expression in relation to the physiological and biochemical parameters. EHL had significantly lower body weight and liver weight at birth, but showed elevated serum levels of total cholesterol (TCH), high-density lipoprotein cholesterol (HDLC) and low-density lipoprotein cholesterol (LDLC), as well as higher liver content of cholesterol. Higher serum cortisol and lower serum insulin and leptin were also observed in EHL piglets. Compared to LW, 30 up-regulated and 18 down-regulated miRNAs were identified in the liver of EHL, together with 298 up-regulated and 510 down-regulated mRNAs (FDR<10%). RT-PCR validation of some differentially expressed miRNAs (DEMs) further confirmed the high-throughput data analysis. Using a target prediction algorithm, we found significant correlation between the up-regulated miRNAs and down-regulated mRNAs. Moreover, differentially expressed genes (DEGs), which are involved in proteolysis, were predicted to be mediated by DEMs. These findings provide new information on the miRNA and mRNA profiles in porcine liver, which would shed light on the molecular mechanisms underlying the breed-specific traits in the pig, and may serve as a basis for further investigation into the biological functions of miRNAs in porcine liver.
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影响因子：
64.8
作者：
Baek, Daehyun;Villen, Judit;Shin, Chanseok;Camargo, Fernando D.;Gygi, Steven P.;Bartel, David P.
通讯作者：
Bartel, David P.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
对骨骼肌生长不同的鸡肉品种的骨骼肌 miRNA 转录组进行系统分析，鉴定出新的 miRNA 和差异表达的 miRNA
影响因子：
4.4
作者：
Li T;Wu R;Zhang Y;Zhu D
通讯作者：
Zhu D
影响因子：
29
作者：
Esau, C;Davis, S;Monia, BP
通讯作者：
Monia, BP
影响因子：
--
作者：
Desjardins, Philippe R;Conklin, Deborah S
通讯作者：
Conklin, Deborah S