Tracking disease resistance deployment in potato breeding by enrichment sequencing.
Tracking disease resistance deployment in potato breeding by enrichment sequencing.
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DOI：
10.1111/pbi.12997
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发表时间：
2019-03
影响因子：
13.8
通讯作者：
Hein I
中科院分区：
文献类型：
--
作者：
Armstrong MR;Vossen J;Lim TY;Hutten RCB;Xu J;Strachan SM;Harrower B;Champouret N;Gilroy EM;Hein I
随着近年来功能抗病基因的分子鉴定，通过抗性叠加进行作物改良需要跟踪和验证品种中多个基因的完整性的方法。诊断性抗性基因富集测序（dRenSeq）可实现作物中已知功能性抗性基因的高置信度鉴定和完整序列验证。正如四倍体马铃薯品种所证明的那样，该方法在监测抗性方面比全基因组测序更稳健和更具成本效益，并且可用于有效地评估（转）基因完整性。所有目前已知的对病毒、线虫和晚疫病病原体致病疫霉有效的NB-LRR都可以在马铃薯中用dRenSeq追踪，并且已经鉴定了迄今未知的多态性。该方法提供了一种通过将亲本和后代选择导向抗性基因的有效组合来提高未来作物抗病育种的速度和效率的手段。
Following the molecular characterisation of functional disease resistance genes in recent years, methods to track and verify the integrity of multiple genes in varieties are needed for crop improvement through resistance stacking. Diagnostic resistance gene enrichment sequencing (dRenSeq) enables the high‐confidence identification and complete sequence validation of known functional resistance genes in crops. As demonstrated for tetraploid potato varieties, the methodology is more robust and cost‐effective in monitoring resistances than whole‐genome sequencing and can be used to appraise (trans) gene integrity efficiently. All currently known NB‐LRRs effective against viruses, nematodes and the late blight pathogen Phytophthora infestans can be tracked with dRenSeq in potato and hitherto unknown polymorphisms have been identified. The methodology provides a means to improve the speed and efficiency of future disease resistance breeding in crops by directing parental and progeny selection towards effective combinations of resistance genes.
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DOI：
10.1093/bioinformatics/btq033
发表时间：
2010-03-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Quinlan AR;Hall IM
通讯作者：
Hall IM
DOI：
10.1111/tpj.12307
发表时间：
2013-11
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Jupe F;Witek K;Verweij W;Sliwka J;Pritchard L;Etherington GJ;Maclean D;Cock PJ;Leggett RM;Bryan GJ;Cardle L;Hein I;Jones JD
通讯作者：
Jones JD
影响因子：
7.2
作者：
van der Vossen, EAG;van der Voort, JNAMR;Klein-Lankhorst, RM
通讯作者：
Klein-Lankhorst, RM
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
5.4
作者：
Kim, Hyoun-Joung;Lee, Heung-Ryul;Jo, Kwang-Ryong;Mortazavian, S. M. Mahdi;Huigen, Dirk Jan;Evenhuis, Bert;Kessel, Geert;Visser, Richard G. F.;Jacobsen, Evert;Vossen, Jack H.
通讯作者：
Vossen, Jack H.