Broad spectrum late blight resistance in potato differential set plants MaR8 and MaR9 is conferred by multiple stacked R genes.
Broad spectrum late blight resistance in potato differential set plants MaR8 and MaR9 is conferred by multiple stacked R genes.
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DOI：
10.1007/s00122-011-1757-7
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发表时间：
2012-03
影响因子：
5.4
通讯作者：
Vossen, Jack H.
中科院分区：
文献类型：
--
作者：
Kim, Hyoun-Joung;Lee, Heung-Ryul;Jo, Kwang-Ryong;Mortazavian, S. M. Mahdi;Huigen, Dirk Jan;Evenhuis, Bert;Kessel, Geert;Visser, Richard G. F.;Jacobsen, Evert;Vossen, Jack H.
致病疫霉是马铃薯晚疫病的病原菌。墨西哥物种Solanum demissum是众所周知的良好抗性来源。在11个R基因差异中，从S.在室内和田间条件下，对demissum的抗性表现出广谱性，特别是R8和R9分化株。为了收集更多关于R8和R9分化的抗性的信息，通过将Mastenbroek（Ma）R8和R9克隆与感病植物杂交来制备F1和BC1群体。父母和后代植物进行了检查，他们的病原体识别特异性使用agroinfiltration与已知的Avr基因，离体叶片测定（DLA）与选定的菌株，和基因特异性标记。一个重要的观察结果是在所有F1和BC1群体中Pi分离物IPO-C的DLA和田间试验结果之间的差异，因此田间试验结果也包括在我们的表征中。结果表明，在MaR8和MaR9中分别存在至少4个（R3a、R3b、R4和R8）和7个（R1、Rpi-abpt 1、R3a、R3b、R4、R8、R9）R基因。MaR8和MaR9后代植物的分析，其中包含不同的组合的多个抗性基因，表明R基因堆叠贡献的Pi识别谱。此外，在田间使用Pi毒力监测系统，表明多个R基因的堆叠强烈延迟了晚疫病症状的发作。R8对这种延迟的贡献是显著的，因为仅含有R8抗性基因的植物仍然赋予与具有多个抗性基因的植物类似的延迟，例如，cv Sarpo Mira.使用这种"去堆积"方法，可以制备和测试许多R基因组合，以便选择广谱R基因堆叠，其潜在地为未来在新的晚疫病抗性品种中的应用提供增强的耐久性。本文的在线版本（doi：10.1007/s00122 - 011 - 1757 - 7）包含补充材料，可供授权用户使用。
Phytophthora infestans is the causal agent of late blight in potato. The Mexican species Solanum demissum is well known as a good resistance source. Among the 11 R gene differentials, which were introgressed from S. demissum, especially R8 and R9 differentials showed broad spectrum resistance both under laboratory and under field conditions. In order to gather more information about the resistance of the R8 and R9 differentials, F1 and BC1 populations were made by crossing Mastenbroek (Ma) R8 and R9 clones to susceptible plants. Parents and offspring plants were examined for their pathogen recognition specificities using agroinfiltration with known Avr genes, detached leaf assays (DLA) with selected isolates, and gene-specific markers. An important observation was the discrepancy between DLA and field trial results for Pi isolate IPO-C in all F1 and BC1 populations, so therefore also field trial results were included in our characterization. It was shown that in MaR8 and MaR9, respectively, at least four (R3a, R3b, R4, and R8) and seven (R1, Rpi-abpt1, R3a, R3b, R4, R8, R9) R genes were present. Analysis of MaR8 and MaR9 offspring plants, that contained different combinations of multiple resistance genes, showed that R gene stacking contributed to the Pi recognition spectrum. Also, using a Pi virulence monitoring system in the field, it was shown that stacking of multiple R genes strongly delayed the onset of late blight symptoms. The contribution of R8 to this delay was remarkable since a plant that contained only the R8 resistance gene still conferred a delay similar to plants with multiple resistance genes, like, e.g., cv Sarpo Mira. Using this “de-stacking” approach, many R gene combinations can be made and tested in order to select broad spectrum R gene stacks that potentially provide enhanced durability for future application in new late blight resistant varieties. The online version of this article (doi:10.1007/s00122-011-1757-7) contains supplementary material, which is available to authorized users.
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影响因子：
1.9
作者：
BLACK, W.;MASTENBROEK, C.;PETERSON, L. C.
通讯作者：
PETERSON, L. C.
影响因子：
7.2
作者：
Huang, SW;van der Vossen, EAG;Visser, RGF
通讯作者：
Visser, RGF
影响因子：
7.2
作者：
Ballvora, A;Ercolano, MR;Gebhardt, C
通讯作者：
Gebhardt, C
影响因子：
1.9
作者：
Bisognin, DA;Douches, DS;Kirk, WW
通讯作者：
Kirk, WW
影响因子：
3.5
作者：
Lokossou, Anoma A.;Park, Tae-ho;van der Vossen, Edwin A. G.
通讯作者：
van der Vossen, Edwin A. G.