Signatures of photo-aging and intrinsic aging in skin were revealed by transcriptome network analysis.
Signatures of photo-aging and intrinsic aging in skin were revealed by transcriptome network analysis.
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DOI：
10.18632/aging.101496
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发表时间：
2018-07-18
期刊：
影响因子：
--
通讯作者：
Seo JS
中科院分区：
文献类型：
--
作者：
Cho BA;Yoo SK;Seo JS
有各种各样的因素会改变皮肤的生理特征。阐明内因和外因转录改变的潜在机制可能有助于我们理解皮肤的衰老过程。为了确定老化皮肤的转录组学变化，我们分析了基因型组织表达（GTEx）项目中公开可用的RNA测序数据。GTEx提供了光保护和光损伤的耻骨上皮肤（n=228）和小腿皮肤（n=349）的RNA测序数据。利用差异表达基因分析和加权基因共表达网络分析，我们表征了紫外线照射和衰老引起的转录组变化。参与皮肤发育的基因，如表皮分化复合物组分（SPRR和LCE家族）、脉管系统发育（TGFBR1、TGFBR2、TGFBR3、KDR、FGF2和VEGFC）和基质金属蛋白酶（MMP2、MMP3、MMP8、MMP10和MMP13）在紫外线照射下上调。此外，光损伤皮肤的脂质代谢和线粒体生物发生也出现下调。此外，随着年龄的增长，耻骨上和小腿的伤口愈合过程普遍下调，脂质代谢的进一步下调和血管发育的上调被发现为光老化的特征。在这项研究中，在衰老皮肤中观察到动态转录组变化。因此，我们的发现可能有助于发现皮肤再生的潜在治疗靶点。
There are various factors that alter physiological characteristics in skin. Elucidating the underlying mechanism of transcriptional alterations by intrinsic and extrinsic factors may lead us to understand the aging process of skin. To identify the transcriptomic changes of the aging skin, we analyzed publicly available RNA sequencing data from Genotype-Tissue Expression (GTEx) project. GTEx provided RNA sequencing data of suprapubic (n=228) and lower leg (n=349) skins, which are photo-protected and photo–damaged. Using differentially expressed gene analysis and weighted gene co-expression network analysis, we characterized transcriptomic changes due to UV exposure and aging. Genes involved in skin development such as epidermal differentiation complex component (SPRR and LCE families), vasculature development (TGFBR1, TGFBR2, TGFBR3, KDR, FGF2, and VEGFC), and matrix metalloproteinase (MMP2, MMP3, MMP8, MMP10, and MMP13) were up-regulated by UV exposure. Also, down-regulated lipid metabolism and mitochondrial biogenesis were observed in photo-damaged skin. Moreover, wound healing process was universally down-regulated in suprapubic and lower leg with aging and further down-regulation of lipid metabolism and up-regulation of vasculature development were found as photo-aging signatures. In this study, dynamic transcriptomic alterations were observed in aged skin. Hence, our findings may help to discover a potential therapeutic target for skin rejuvenation.
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发表时间：
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