Self assembled bi-functional peptide hydrogels with biomineralization-directing peptides.
Self assembled bi-functional peptide hydrogels with biomineralization-directing peptides.
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DOI：
10.1016/j.biomaterials.2010.06.010
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发表时间：
2010-10
期刊：
影响因子：
14
通讯作者：
Sarikaya M
中科院分区：
文献类型：
--
作者：
Gungormus M;Branco M;Fong H;Schneider JP;Tamerler C;Sarikaya M
已经设计了一种基于肽的水凝胶，其指导羟基磷灰石的形成。MDG 1是一种27个残基的肽，其经历触发折叠以形成不对称的β-发夹，其响应于溶液离子强度的增加而自组装以产生机械刚性的自支撑水凝胶。MDG 1的C-末端部分含有能够指导矿化过程的七肽（MLPHHGA）。圆二色性光谱表明肽折叠并组装形成富含β-折叠二级结构的水凝胶网络。振荡流变学表明，在矿化之前，水凝胶是机械刚性的（G′ = 2500 Pa）。在单独的实验中，矿化诱导生化和成牙骨质细胞。矿化域对凝胶的机械刚度影响不大。SEM和EDS表明MDG 1凝胶能够引导羟基磷灰石的形成。控制水凝胶，制备肽缺乏矿物质导向部分或逆转其序列，表明七肽是必要的，其行动是序列特异性的。
A peptide-based hydrogel has been designed that directs the formation of hydroxyapatite. MDG1, a twenty-seven residue peptide, undergoes triggered folding to form an unsymmetrical β-hairpin that self-assembles in response to an increase in solution ionic strength to yield a mechanically rigid, self supporting hydrogel. The C-terminal portion of MDG1 contains a heptapeptide (MLPHHGA) capable of directing the mineralization process. Circular dichroism spectroscopy indicates that the peptide folds and assembles to form a hydrogel network rich in β-sheet secondary structure. Oscillatory rheology indicates that the hydrogel is mechanical rigid (G′ ∼ 2500 Pa) before mineralization. In separate experiments, mineralization was induced both biochemically and with cementoblast cells. Mineralization-domain had little effect on the mechanical rigidity of the gel. SEM and EDS show that MDG1 gels are capable of directing the formation of hydroxapatite. Control hydrogels, prepared by peptides either lacking the mineral-directing portion or reversing its sequence, indicated that the heptapeptide is necessary and its actions are sequence specific.
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DOI：
10.1073/pnas.0701980104
发表时间：
2007-05-08
影响因子：
11.1
作者：
Haines-Butterick, Lisa;Rajagopal, Karthikan;Schneider, Joel P.
通讯作者：
Schneider, Joel P.
DOI：
10.1073/pnas.142309999
发表时间：
2002-07-23
影响因子：
11.1
作者：
Kisiday, J;Jin, M;Grodzinsky, AJ
通讯作者：
Grodzinsky, AJ
影响因子：
6.2
作者：
Gungormus, Mustafa;Fong, Hanson;Sarikaya, Mehmet
通讯作者：
Sarikaya, Mehmet
影响因子：
--
作者：
Bhatnagar, RS;Qian, JJ;Smith, N
通讯作者：
Smith, N
影响因子：
14
作者：
Kretsinger, JK;Haines, LA;Schneider, JP
通讯作者：
Schneider, JP