Circadian clock NAD+ cycle drives mitochondrial oxidative metabolism in mice.
Circadian clock NAD+ cycle drives mitochondrial oxidative metabolism in mice.
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DOI：
10.1126/science.1243417
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发表时间：
2013-11-01
期刊：
影响因子：
--
通讯作者：
Bass J
中科院分区：
文献类型：
--
作者：
Peek CB;Affinati AH;Ramsey KM;Kuo HY;Yu W;Sena LA;Ilkayeva O;Marcheva B;Kobayashi Y;Omura C;Levine DC;Bacsik DJ;Gius D;Newgard CB;Goetzman E;Chandel NS;Denu JM;Mrksich M;Bass J
昼夜节律钟是自我维持的细胞振荡器，其在太阳周期的预期下同步氧化和还原循环。我们发现，时钟转录反馈回路通过调节线粒体蛋白乙酰化，使氧化代谢途径与24小时禁食和进食周期同步，从而产生烟酰胺腺嘌呤二核苷酸（NAD+）生物合成、三磷酸腺苷产生和线粒体呼吸的循环。NAD+依赖性去乙酰化酶sirtuin 3（SIRT3）活性的昼夜节律控制在分离的线粒体中产生乙酰化和氧化酶活性以及呼吸的节律，NAD+补充恢复了昼夜节律突变小鼠的蛋白质去乙酰化并增强了氧消耗。因此，NAD+生物利用度的昼夜节律控制在禁食和进食的每日周期中调节线粒体氧化功能和生物体代谢。
Circadian clocks are self-sustained cellular oscillators that synchronize oxidative and reductive cycles in anticipation of the solar cycle. We found that the clock transcription feedback loop produces cycles of nicotinamide adenine dinucleotide (NAD+) biosynthesis, adenosine triphosphate production, and mitochondrial respiration through modulation of mitochondrial protein acetylation to synchronize oxidative metabolic pathways with the 24-hour fasting and feeding cycle. Circadian control of the activity of the NAD+-dependent deacetylase sirtuin 3 (SIRT3) generated rhythms in the acetylation and activity of oxidative enzymes and respiration in isolated mitochondria, and NAD+ supplementation restored protein deacetylation and enhanced oxygen consumption in circadian mutant mice. Thus, circadian control of NAD+ bioavailability modulates mitochondrial oxidative function and organismal metabolism across the daily cycles of fasting and feeding.
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影响因子：
64.8
作者：
Alenghat, Theresa;Meyers, Katherine;Mullican, Shannon E.;Leitner, Kirstin;Adeniji-Adele, Adetoun;Avila, Jacqueline;Bucan, Maja;Ahima, Rexford S.;Kaestner, Klaus H.;Lazar, Mitchell A.
通讯作者：
Lazar, Mitchell A.
SIRT3在饮食限制期间促进尿素周期和脂肪酸氧化。
影响因子：
16
作者：
Hallows WC;Yu W;Smith BC;Devries MK;Ellinger JJ;Someya S;Shortreed MR;Prolla T;Markley JL;Smith LM;Zhao S;Guan KL;Denu JM
通讯作者：
Denu JM
影响因子：
4.5
作者：
Liu AC;Tran HG;Zhang EE;Priest AA;Welsh DK;Kay SA
通讯作者：
Kay SA
影响因子：
14.9
作者：
Pizarro A;Hayer K;Lahens NF;Hogenesch JB
通讯作者：
Hogenesch JB
DOI：
10.1126/science.1171641
发表时间：
2009-05-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ramsey KM;Yoshino J;Brace CS;Abrassart D;Kobayashi Y;Marcheva B;Hong HK;Chong JL;Buhr ED;Lee C;Takahashi JS;Imai S;Bass J
通讯作者：
Bass J