New insights into the role of MADS-box transcription factor gene CmANR1 on root and shoot development in chrysanthemum (Chrysanthemum morifolium).
New insights into the role of MADS-box transcription factor gene CmANR1 on root and shoot development in chrysanthemum (Chrysanthemum morifolium).
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MADS-box转录因子基因CmANR1对菊花根和芽发育作用的新见解
DOI：
10.1186/s12870-021-02860-7
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发表时间：
2021-02-06
影响因子：
5.3
通讯作者：
Ma F
中科院分区：
文献类型：
--
作者：
Sun CH;Wang JH;Gu KD;Zhang P;Zhang XY;Zheng CS;Hu DG;Ma F
背景
MADS-box转录因子是植物多种发育过程中的关键调控因子，其中菊花MADS-box转录因子Tf CmANR1已被分离出来，并被描述为响应高硝酸盐浓度信号而在根发育中发挥功能。然而，CmANR1如何影响根和地上部的发育仍不清楚。
结果
我们报道了在菊花组织培养的整个发育阶段，CmANR1在菊花根系发育中起着积极的作用。代谢组学结合转录组学分析表明，CmANR1通过促进硝酸盐的同化，并影响氨基酸、糖酵解和三羧酸循环(TCA)的代谢途径，促进了根系的旺盛发育。此外，我们还发现，与硝酸盐信号通路相关的转录因子，如AGL8、AGL21和LBD29，在CmANR1转基因植株中的表达水平显著高于野生型(WT)对照；相比之下，RHD3-like、LBD37和GATA23的表达水平显著下调。这些结果表明，这些与硝酸盐信号相关的转录因子参与了CmANR1对根发育的调控。此外，CmANR1还作为一种正向调节因子来控制新梢的生长发育。
结论
这些发现提供了MADS-box Tf CmANR1调控根和地上部发育的潜在机制，这是通过调节一系列与硝酸盐信号相关的Tf，影响氨基酸和糖酵解的代谢途径，以及TCA循环和硝酸盐同化来实现的。
Background
MADS-box transcription factors (TFs) are the key regulators of multiple developmental processes in plants; among them, a chrysanthemum MADS-box TF CmANR1 has been isolated and described as functioning in root development in response to high nitrate concentration signals. However, how CmANR1 affects root and shoot development remains unclear.
Results
We report that CmANR1 plays a positive role in root system development in chrysanthemum throughout the developmental stages of in vitro tissue cultures. Metabolomics combined with transcriptomics assays show that CmANR1 promotes robust root system development by facilitating nitrate assimilation, and influencing the metabolic pathways of amino acid, glycolysis, and the tricarboxylic acid cycle (TCA) cycle. Also, we found that the expression levels of TFs associated with the nitrate signaling pathways, such as AGL8, AGL21, and LBD29, are significantly up-regulated in CmANR1-transgenic plants relative to the wild-type (WT) control; by contrast, the expression levels of RHD3-LIKE, LBD37, and GATA23 were significantly down-regulated. These results suggest that these nitrate signaling associated TFs are involved in CmANR1-modulated control of root development. In addition, CmANR1 also acts as a positive regulator to control shoot growth and development.
Conclusions
These findings provide potential mechanisms of MADS-box TF CmANR1 modulation of root and shoot development, which occurs by regulating a series of nitrate signaling associated TFs, and influencing the metabolic pathways of amino acid and glycolysis, as well as TCA cycle and nitrate assimilation.
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