Osteopontin: an early innate immune marker of Escherichia coli mastitis harbors genetic polymorphisms with possible links with resistance to mastitis.
Osteopontin: an early innate immune marker of Escherichia coli mastitis harbors genetic polymorphisms with possible links with resistance to mastitis.
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DOI：
10.1186/1471-2164-10-444
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发表时间：
2009-09-18
期刊：
影响因子：
4.4
通讯作者：
Bissonnette N
中科院分区：
文献类型：
--
作者：
Alain K;Karrow NA;Thibault C;St-Pierre J;Lessard M;Bissonnette N
乳腺炎是奶牛最重要的疾病之一，给奶牛生产者带来了巨大的经济损失。识别参与先天免疫反应的基因及其变体对于理解这种炎性疾病和识别抵抗乳腺炎的潜在遗传标记至关重要。奶牛的后代将受益于接受有利的等位基因，这些等位基因支持对感染的更大抵抗力，从而减少抗生素的使用。本研究旨在确定对乳腺炎的先天免疫反应中的关键基因，使我们评估其与体细胞评分（SCS）的遗传相关性，这是临床乳腺炎的指标，并评估其对其他与产奶相关性状的影响。在实验感染大肠杆菌的奶牛体细胞中鉴定了骨桥蛋白转录本（SPP 1）。通过选择具有SCS极端估计育种值（EBV）的公牛，发现SPP 1基因组序列中的四个DNA多态性。1301 G>A对SCS的EBV有影响（P < 0.001）。1251C>T，SPP 1c.- 430G>A和SPP 1c。40 A>C对SCS有影响，而SPP 1c.- 1301 G>A对产奶量（第二次和第三次哺乳）、脂肪和蛋白质百分比（所有三次哺乳）的EBV有影响。分析显示，在与父系EBVS比较的水平上，单倍型组之间在第一次（P = 0.012）、第二次（P < 0.001）和第三次（P < 0.001）泌乳的SCS方面存在统计学显著差异。这项研究报告了SPP 1的DNA多态性，牛奶免疫细胞的数量和潜在的乳腺炎易感性之间的联系。这些SNP通过计算机搜索被鉴定为位于转录因子识别位点，这些转录因子识别位点可能参与Th 1免疫应答和Th 2调节途径。事实上，一个SNP废除了SP1识别位点，而另一个SNP影响转录结合因子IKAROS。总之，这些发现支持了这些变体在选择提高奶牛乳腺炎抗性方面的遗传潜力。
Mastitis is the most important disease in dairy cows and it causes significant lost of profit to producers. Identification of the genes, and their variants, involved in innate immune responses is essential for the understanding of this inflammatory disease and to identify potential genetic markers for resistance to mastitis. The progeny of dairy cows would benefit from receiving favourable alleles that support greater resistance to infection, thus reducing antibiotic use. This study aims to identify a key gene in the innate immune response to mastitis, led us to evaluate its genetic association with somatic cell score (SCS), which is an indicator of clinical mastitis, and to evaluate its impact on other traits related to milk production. The osteopontin transcript (SPP1) was identified in the somatic cells from cows experimentally infected with Escherichia coli. By selecting bulls with extreme estimated breeding values (EBVs) for SCS, which is an indicator of mammary gland health, four DNA polymorphisms in the SPP1 genomic sequence were found. Statistical analysis revealed that the SNP SPP1c.-1301G>A has an impact on EBV for SCS (P < 0.001) Using an allele substitution model, SPP1c.-1251C>T, SPP1c.-430G>A, and SPP1c.*40A>C have an impact on SCS whereas SPP1c.-1301G>A has an effect on the EBVs for milk yield (second and third lactations), fat and protein percentages (all three lactations). Analysis revealed statistically significant differences between haplotype groups at a comparison-wise level with sire EBVS for SCS for the first (P = 0.012), second (P < 0.001), and third (P < 0.001) lactations. This study reports the link between DNA polymorphisms of SPP1, the number of milk immune cells and, potentially, the susceptibility to mastitis. These SNPs were identified by in silico search to be located in transcription factor recognition sites which factors are presumably involved in the Th1 immune response and in the Th2 regulation pathway. Indeed, one SNP abolished the SP1 recognition site, whereas another SNP affected the transcription binding factor IKAROS. All together, these findings support the genetic potential of these variants in terms of selection for the improvement of mastitis resistance in dairy cows.
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影响因子：
3.5
作者：
Begley, N.;Buckley, F.;Mallard, B. A.
通讯作者：
Mallard, B. A.
影响因子：
3.5
作者：
Heringstad, B.;Sehested, E.;Steine, T.
通讯作者：
Steine, T.
影响因子：
4.2
作者：
Campbell, TM;Wong, WT;Mackie, EJ
通讯作者：
Mackie, EJ
DOI：
10.1073/pnas.91.1.192
发表时间：
1994-01-04
影响因子：
11.1
作者：
GILL, G;PASCAL, E;TJIAN, R
通讯作者：
TJIAN, R
DOI：
10.1111/j.1749-6632.1995.tb44624.x
发表时间：
1995-01-01
期刊：
OSTEOPONTIN: ROLE IN CELL SIGNALLING AND ADHESION
影响因子：
--
作者：
GIACHELLI, CM;LIAW, L;ALMEIDA, M
通讯作者：
ALMEIDA, M