A Cotton Gbvdr5 Gene Encoding a Leucine-Rich-Repeat Receptor-Like Protein Confers Resistance to Verticillium dahliae in Transgenic Arabidopsis and Upland Cotton
A Cotton Gbvdr5 Gene Encoding a Leucine-Rich-Repeat Receptor-Like Protein Confers Resistance to Verticillium dahliae in Transgenic Arabidopsis and Upland Cotton
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编码富含亮氨酸重复受体样蛋白的棉花 Gbvdr5 基因赋予转基因拟南芥和陆地棉对大丽黄萎病的抗性
DOI：
10.1007/s11105-014-0810-5
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发表时间：
2015-08
影响因子：
2.1
通讯作者：
Ma Din-Pow
中科院分区：
文献类型：
--
作者：
Yang Yuwen;Ling Xitie;Chen Tianzi;Cai Liwang;Liu Tingli;Wang Jinyan;Fan Xiaohui;Ren Yongzhe;Yuan Hongbo;Zhu Wei;Zhang Baolong;Ma Din-Pow
富含亮氨酸重复序列的受体样蛋白（Leucine-rich-repeat receptor-like proteins，eLRR-RLP）在植物防御病原菌和植物发育中起着重要作用。已经报道了几个eLRR-RLP基因如Ve 1、Gbve和Gbve 1赋予对大丽轮枝菌的抗性。Gbvdr 5是从海岛棉抗黄萎病品种H7124中发现的一个新的RFLP基因，与Ve 1一样，具有短尾。所有感病高地棉品种Gbvdr 5同源基因起始密码子下游2765位核苷酸均存在胞嘧啶缺失。发现这种缺失导致蛋白质的过早终止，产生937个氨基酸的产物，但Gbvdr 5蛋白具有完整的1，077个氨基酸。瞬时表达分析表明Gbvdr 5定位于质膜上。定量聚合酶链反应（PCR）分析表明，Gbvdr 5基因在H7124中被SA、MeJA、阿坝和ETH激活，并被V. dahliae分离物V991和DF-CQ-2诱导，而在敏感高地棉泗棉3号中没有变化或被抑制。Gbvdr 5启动子驱动的GUS活性主要存在于转基因拟南芥的根尖和茎生长点。抗黄萎病棉花H7124中Gbvdr 5的沉默损害了棉花对大丽轮枝菌分离物V991和BP 2的抗性。通过农杆菌介导法将Gbvdr 5基因转化拟南芥和高地棉，验证了Gbvdr 5基因的抗性。Gbvdr 5基因的过表达使转基因拟南芥对落叶型菌株V991和非落叶型菌株BP 2具有抗性，但对大丽轮枝菌的DF-CQ-2和JR 2没有影响。转化的棉花还具有对V991和BP 2的确认抗性。在接种大丽轮枝菌的转基因拟南芥中观察到更多的胼胝质沉积，更多的防御相关基因PR 1和PR 5的表达，以及HR模拟细胞死亡。这表明黄萎病菌与植物的互作可能涉及到一些特异的识别方式，Gbvdr 5基因可能是培育抗黄萎病棉花品系的候选基因。
Leucine-rich-repeat receptor-like proteins (eLRR-RLPs) play significant roles in plant defense against pathogens and in plant development. Several eLRR-RLP genes such as Ve1, Gbve, and Gbve1 have been reported to confer resistance to Verticillium dahliae. Gbvdr5, a newly discovered RLP gene from V. dahliae-resistant island cotton cultivar H7124, has a short tail as Ve1. There is a cytosine deletion in Gbvdr5 homologous genes at nucleotide position 2765, which is downstream from the initiation codon in all susceptible upland cotton cultivars analyzed. This deletion was found to cause premature termination of the protein, creating a 937 aa product, but the Gbvdr5 protein had the full 1,077 aa. Transient expression analyses indicated that Gbvdr5 is localized on the plasma membrane. Quantitative polymerase chain reaction analysis revealed that the Gbvdr5 gene was activated by SA, MeJA, ABA, and ETH, and it was induced by V. dahliae isolates V991 and DF-CQ-2 in H7124, whereas was unchanged or repressed in susceptible upland cotton Simian 3. Gbvdr5-promoter-driven GUS activity was found mostly in the root tips and stem growing points of transgenic Arabidopsis. Silencing of Gbvdr5 in Verticillium-wilt-resistant cotton H7124 compromised cotton resistance to V. dahliae isolates V991 and BP2. The resistance was verified by transforming the Gbvdr5 gene into Arabidopsis and upland cotton through Agrobacterium-mediated transformation. Overexpression of the Gbvdr5 gene endowed transgenic Arabidopsis with resistance to defoliating isolate V991 and non-defoliating isolate BP2, but it had no effect on either DF-CQ-2 or JR2 of V. dahliae. The transformed cotton also had confirmed resistance to V991 and BP2. More callose deposition, more expression of the defense-related genes PR1 and PR5, and HR-mimic cell death were observed in the transgenic Arabidopsis when inoculated with V. dahliae. This demonstrated that Verticillium–plant interactions may involve some specific ways of recognizing V. dahliae and Gbvdr5 may be a suitable candidate gene for breeding Verticillium-wilt-resistant cotton lines.
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影响因子：
11.6
作者：
Ron, M;Avni, A
通讯作者：
Avni, A
影响因子：
1.2
作者：
K. Zuo;Jin Wang;Weisheng Wu;Y. Chai;Xiaofen Sun;K. Tang
通讯作者：
K. Zuo;Jin Wang;Weisheng Wu;Y. Chai;Xiaofen Sun;K. Tang
影响因子：
4.9
作者：
Zhao Zhang;H. P. van Esse;M. van Damme;E. Fradin;Chun-Ming Liu;B. Thomma
通讯作者：
Zhao Zhang;H. P. van Esse;M. van Damme;E. Fradin;Chun-Ming Liu;B. Thomma
影响因子：
3.5
作者：
K. Zuo;J. Qin;Jing-ya Zhao;H. Ling;Lida Zhang;Youfang Cao;K. Tang
通讯作者：
K. Zuo;J. Qin;Jing-ya Zhao;H. Ling;Lida Zhang;Youfang Cao;K. Tang
DOI：
10.1094/mpmi-06-12-0161-r
发表时间：
2013-01
期刊：
Molecular plant-microbe interactions : MPMI
影响因子：
--
作者：
Zhao Zhang;E. Fradin;R. de Jonge;H. P. van Esse;P. Smit;Chun-Ming Liu;B. Thomma
通讯作者：
Zhao Zhang;E. Fradin;R. de Jonge;H. P. van Esse;P. Smit;Chun-Ming Liu;B. Thomma