Functional recovery after complete contusion injury to the spinal cord and transplantation of human neuroteratocarcinoma neurons in rats.
Functional recovery after complete contusion injury to the spinal cord and transplantation of human neuroteratocarcinoma neurons in rats.
复制标题
大鼠脊髓完全挫伤和人神经畸胎瘤神经元移植后的功能恢复。
DOI：
10.3171/spi.2002.97.1.0063
复制
发表时间：
2002
影响因子：
4.1
通讯作者：
Paul R. Sanberg
中科院分区：
文献类型：
--
作者：
S. Saporta;A. Shahram Makoui;A. Willing;M. Daadi;David W. Cahill;Paul R. Sanberg
对象
人神经畸胎癌(HNT)来源的神经元是分化的有丝分裂后神经元，来自人类畸胎癌细胞系，经维甲酸处理后。在临床前移植研究中，研究人员证明了它们作为移植神经元来源的安全性，以及在治疗中风相关行为缺陷方面的有效性。这项研究的目的是检查HNT神经元移植到完全脊髓挫伤区是否会改善脊髓功能的电生理指标。
方法
在T-8时，30只大鼠造成脊髓完全挫伤，定义为运动诱发电位(MEPs)的完全丧失。10只大鼠脊髓挫伤后立即在脊髓挫伤处移植HNT神经元(即刻移植组)。在为期两周的MEP丢失评估后，10只大鼠接受了HNT神经元移植(延迟移植组)。另10只挫伤大鼠作为脊髓损伤对照组，10只大鼠仅行T-8椎板切除，作为非损伤对照组。所有大鼠在移植后8周存活。在延迟移植组，观察到显著的功能恢复，表现为MEP的恢复和运动功能的轻微改善。免疫组织化学分析显示移植的HNT神经元存活、整合和长纤维生长。
结论
这些结果提示，移植HNT神经元可能是重建受损脊髓电连接的有效手段。
OBJECT
Human neuroteratocarcinoma (hNT)-derived neurons are differentiated postmitotic neurons derived from a human teratocarcinoma cell line following treatment with retinoic acid. In preclinical transplantation studies investigators have demonstrated both their safety as a source of neurons for transplantation and efficacy in treating stroke-related behavioral deficits. The objective of this study was to examine whether hNT neurons transplanted in an area of complete spinal cord contusion would improve electrophysiological measures of spinal cord function.
METHODS
Complete spinal cord contusion injury, defined as the complete loss of motor evoked potentials (MEPs), was produced in 30 rats at T-8. Ten rats with contused spinal cords underwent transplantation with hNT neurons within the site of contusion immediately after injury (immediate transplant group). Ten rats underwent hNT neuron transplantation following a 2-week evaluation for loss of MEPs (delayed transplant group). Ten other rats with contusion injury served as a spinal cord injury control group, and 10 rats underwent only a T-8 laminectomy and served as non-injured controls. All rats survived 8 weeks after transplantation. In the delayed transplant group significant functional recovery was observed, as demonstrated by return of MEPs and a modest improvement of motor function. Immunohistochemical analysis showed the survival, integration, and long fiber outgrowth of the grafted hNT neurons.
CONCLUSIONS
These findings suggest that the transplantation of the hNT neurons may be an effective means of reestablishing electrical connectivity of the injured spinal cord.
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影响因子：
4.1
作者：
Matthew F. Philips;J. K. Muir;K. Saatman;R. Raghupathi;V. Lee;J. Trojanowski;Tracy K. McIntosh
通讯作者：
Matthew F. Philips;J. K. Muir;K. Saatman;R. Raghupathi;V. Lee;J. Trojanowski;Tracy K. McIntosh
DOI：
10.1073/pnas.97.11.6126
发表时间：
2000-05-23
影响因子：
11.1
作者：
Liu, S;Qu, Y;McDonald, JW
通讯作者：
McDonald, JW
影响因子：
2.7
作者：
ANDREWS, PW
通讯作者：
ANDREWS, PW
神经组织移植和中枢神经系统创伤：受伤脊髓的解剖和功能修复。
影响因子：
4.2
作者：
Reier,PJ;Anderson,DK;Thompson,FJ;Stokes,BT
通讯作者：
Stokes,BT