Regulation of bacteria population behaviors by AI-2 "consumer cells" and "supplier cells".
Regulation of bacteria population behaviors by AI-2 "consumer cells" and "supplier cells".
复制标题
AI-2“消费者细胞”和“供应细胞”对细菌群体行为的调节
DOI：
10.1186/s12866-017-1107-2
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发表时间：
2017-09-19
期刊：
影响因子：
4.2
通讯作者：
Song C
中科院分区：
文献类型：
--
作者：
Quan Y;Meng F;Ma X;Song X;Liu X;Gao W;Dang Y;Meng Y;Cao M;Song C
Autoinducer-2 (AI-2) is a universal signal molecule and enables an individual bacteria to communicate with each other and ultimately control behaviors of the population. Harnessing the character of AI-2, two kinds of AI-2 “controller cells” (“consumer cells” and “supplier cells”) were designed to “reprogram” the behaviors of entire population. For the consumer cells, genes associated with the uptake and processing of AI-2, which includes LsrACDB, LsrFG, LsrK, were overexpressed in varying combinations. Four consumer cell strains were constructed: Escherichia coli MG1655 pLsrACDB (NK-C1), MG1655 pLsrACDBK (NK-C2), MG1655 pLsrACDBFG (NK-C3) and MG1655 pLsrACDBFGK (NK-C4). The key enzymes responsible for production of AI-2, LuxS and Mtn, were also overexpressed, yielding strains MG1655 pLuxS (NK-SU1), and MG1655 pLuxS-Mtn (NK-SU2). All the consumer cells could decrease the environmental AI-2 concentration. NK-C2 and NK-C4 were most effective in AI-2 uptake and inhibited biofilm formation. While suppliers can increase the environmental AI-2 concentration and NK-SU2 was most effective in supplying AI-2 and facilitated biofilm formation. Further, reporter strain, MG1655 pLGFP was constructed. The expression of green fluorescent protein (GFP) in reporter cells was initiated and guided by AI-2. Mixture of consumer cells and reporter cells suggest that consumer cells can decrease the AI-2 concentration. And the supplier cells were co-cultured with reporter cells, indicating that supplier cells can provide more AI-2 compared to the control. The consumer cells and supplier cells could be used to regulate environmental AI-2 concentration and the biofilm formation. They can also modulate the AI-2 concentration when they were co-cultured with reporter cells. It can be envisioned that this system will become useful tools in synthetic biology and researching new antimicrobials. The online version of this article (10.1186/s12866-017-1107-2) contains supplementary material, which is available to authorized users.
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影响因子：
4.4
作者：
Auger, S;Krin, E;Gohar, M
通讯作者：
Gohar, M
影响因子：
3.2
作者：
Barrios, AFG;Zuo, RJ;Wood, TK
通讯作者：
Wood, TK
影响因子：
3.6
作者：
Taga, ME;Miller, ST;Bassler, BL
通讯作者：
Bassler, BL
影响因子：
5.2
作者：
Semmelhack, MF;Campagna, SR;Bassler, BL
通讯作者：
Bassler, BL
影响因子：
4
作者：
Xavier, Karina B.;Miller, Stephen T.;Bassler, Bonnie L.
通讯作者：
Bassler, Bonnie L.