Mouse pancreas tissue slice culture facilitates long-term studies of exocrine and endocrine cell physiology in situ.
Mouse pancreas tissue slice culture facilitates long-term studies of exocrine and endocrine cell physiology in situ.
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DOI：
10.1371/journal.pone.0078706
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Speier S
中科院分区：
文献类型：
--
作者：
Marciniak A;Selck C;Friedrich B;Speier S
胰腺细胞生理学的研究依赖于体外分泌细胞和内分泌细胞的研究。特别是，就外分泌组织而言，这些研究发现培养物中腺泡细胞的功能活力降低。结果，不仅对分散的腺泡细胞和分离的腺泡的研究潜力受到限制，而且在完整的胰腺组织环境中对胰腺外分泌和内分泌细胞进行长期研究也是不可行的。为了克服这些限制，我们的目标是建立一个胰腺组织切片培养平台，以便在完整的胰腺环境中对外分泌和内分泌细胞进行长期研究。评估小鼠胰腺组织切片形态以确定完整胰腺组织的最佳长期培养设置。利用优化的培养条件，在 7 天的培养期内对外分泌腺泡细胞和内分泌 β 细胞的细胞特异性和功能进行了表征。我们发现在优化条件下培养的胰腺组织切片在整个培养期间具有完整的组织特异性形态。淀粉酶阳性的完整腺泡存在于培养的所有时间点，并且腺泡细胞表现出典型的强细胞极性。在培养过程中，胰腺组织切片的淀粉酶释放量减少，但随着雨蛙素浓度的增加，保持了特征性的钟形剂量反应曲线。最大释放量是基础释放量的 4 倍。此外，内分泌β细胞的活力和功能一直保持良好，直到观察期结束。我们的结果表明，组织切片培养平台在至少 4 天、部分甚至长达 1 周的培养期内提供了前所未有的胰腺组织特异性形态和功能的维持。这一分析进展现在允许在完整的原位环境中对胰腺疾病发病机制和治疗的细胞生物学进行中长期研究。
Studies on pancreatic cell physiology rely on the investigation of exocrine and endocrine cells in vitro. Particularly, in the case of the exocrine tissue these studies have suffered from a reduced functional viability of acinar cells in culture. As a result not only investigations on dispersed acinar cells and isolated acini were limited in their potential, but also prolonged studies on pancreatic exocrine and endocrine cells in an intact pancreatic tissue environment were unfeasible. To overcome these limitations, we aimed to establish a pancreas tissue slice culture platform to allow long-term studies on exocrine and endocrine cells in the intact pancreatic environment. Mouse pancreas tissue slice morphology was assessed to determine optimal long-term culture settings for intact pancreatic tissue. Utilizing optimized culture conditions, cell specificity and function of exocrine acinar cells and endocrine beta cells were characterized over a culture period of 7 days. We found pancreas tissue slices cultured under optimized conditions to have intact tissue specific morphology for the entire culture period. Amylase positive intact acini were present at all time points of culture and acinar cells displayed a typical strong cell polarity. Amylase release from pancreas tissue slices decreased during culture, but maintained the characteristic bell-shaped dose-response curve to increasing caerulein concentrations and a ca. 4-fold maximal over basal release. Additionally, endocrine beta cell viability and function was well preserved until the end of the observation period. Our results show that the tissue slice culture platform provides unprecedented maintenance of pancreatic tissue specific morphology and function over a culture period for at least 4 days and in part even up to 1 week. This analytical advancement now allows mid -to long-term studies on the cell biology of pancreatic disorder pathogenesis and therapy in an intact surrounding in situ.
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影响因子：
2.9
作者：
Chen, Nancy;Unnikrishnan, Ranjit, I;Pitchumoni, Capecomorin S.
通讯作者：
Pitchumoni, Capecomorin S.
影响因子：
5.5
作者：
Huang, Ya-Chi;Rupnik, Marjan;Gaisano, Herbert Y.
通讯作者：
Gaisano, Herbert Y.
影响因子：
29.4
作者：
Houbracken, Isabelle;de Waele, Evelien;Bouwens, Luc
通讯作者：
Bouwens, Luc
DOI：
10.1152/ajpendo.00321.2002
发表时间：
2003-01-01
影响因子：
5.1
作者：
Hara, M;Wang, XY;Bell, GI
通讯作者：
Bell, GI
DOI：
10.1085/jgp.200609604
发表时间：
2007-06
期刊：
The Journal of general physiology
影响因子：
--
作者：
Rose T;Efendic S;Rupnik M
通讯作者：
Rupnik M