Nucleotide sequence and some biological properties of the NS gene of a newly isolated influenza B virus mutant which has a long carboxyl terminal deletion in the NS, protein
Nucleotide sequence and some biological properties of the NS gene of a newly isolated influenza B virus mutant which has a long carboxyl terminal deletion in the NS, protein
复制标题
新分离的乙型流感病毒突变体NS基因的核苷酸序列和一些生物学特性，该突变体在NS、蛋白中具有长羧基末端缺失
DOI：
10.1016/0042-6822(90)90082-3
复制
发表时间：
1990
期刊：
影响因子：
3.7
通讯作者：
S. Feng
中科院分区：
文献类型：
--
作者：
K. Tobita;Toshinori Tanaka;T. Odagiri;M. Tashiro;S. Feng
通过流感病毒A/WSN(H1N1)和B/Yamagata/1/73的异型杂交，我们获得了B/Yamagata突变株(AWBY-234)，该突变株表达截短较大的NS蛋白，相对分子质量为13,500。对突变体的NS基因进行直接测序发现，在正义RNA的310、311或312位缺失了一个尿苷碱基，在314-316位产生了一个新的终止密码子。野生型B/Yamagata的NS蛋白仅由90个氨基酸组成，而野生型B/Yamagata的NS蛋白由281个氨基酸组成。AWBY-234在感染MDCK细胞后生长正常，并且比野生型B/Yamagata更早地在感染的MDCK细胞中引起典型的细胞病变效应。将AWBY-234的NS基因转入B/Lee/40的单基因重组体继承了AWBY-234亲本的这些特性。单基因重组体，而不是对照重组体，含有大量的缺陷颗粒，这些缺陷颗粒可以相互补充，产生传染性病毒。
By a heterotypic cross between influenza viruses A/WSN (H1 N1) and B/Yamagata/1/73, we obtained a mutant of B/Yamagata (AWBY-234), which expressed a greatly truncated NS, protein with molecular weight of 13,500. Direct sequencing of the NS gene of the mutant revealed a deletion of a single uridine base at the position 310, 311, or 312 of the plus sense RNA, giving rise to a new stop codon at the position 314–316. The resulting NS, protein was predicted to be composed of only 90 amino acids, compared with 281 with the NS, of the wild-type B/Yamagata.AWBY-234 grew normally and induced a typical cytopathic effect in infected MDCK cells much earlier after infection than did the wild-type B/Yamagata. A single gene reassortant in which the NS gene of AWBY-234 was transferred to B/Lee/40 inherited these characteristics from AWBY-234 parent. The single gene reassortant, but not the control reassortant, contained a significant amount of defective particles which can complement each other to produce infectious virus.
与甲型流感细胞质内含物相关的 RNA 的表征以及 NS1 蛋白与 RNA 的相互作用。
影响因子：
3.7
作者：
Yoshida,T;Shaw,MW;Young,JF;Compans,RW
通讯作者：
Compans,RW
影响因子：
3.7
作者：
G. Norton;T. Tanaka;K. Tobita;S. Nakada;D. Buonagurio;D. Greenspan;M. Krystal;P. Palese
通讯作者：
G. Norton;T. Tanaka;K. Tobita;S. Nakada;D. Buonagurio;D. Greenspan;M. Krystal;P. Palese