Striking similarity in the gene expression levels of individual Myc module members among ESCs, EpiSCs, and partial iPSCs.
Striking similarity in the gene expression levels of individual Myc module members among ESCs, EpiSCs, and partial iPSCs.
复制标题
DOI：
10.1371/journal.pone.0083769
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Okuda A
中科院分区：
文献类型：
--
作者：
Hirasaki M;Hiraki-Kamon K;Kamon M;Suzuki A;Katano M;Nishimoto M;Okuda A
主要的转录亚网络称为核心、Myc和PRC模块，已被证明参与了胚胎干细胞(ESCs)的多能性和自我更新性的保存。表皮干细胞是另一种具有多潜能和自我更新能力的细胞类型。然而，到目前为止，这些模块在EpiSCs中的作用还没有得到系统的研究。在这里，我们比较了ESCs和EpiSCs之间核心、Myc和PRC模块基因的平均表达水平。与ESCs相比，EpiSCs的PRC和Core模块基因的表达水平分别显著升高和降低，而Myc模块成员的平均基因表达水平几乎相同。随后的分析表明，这两种细胞类型之间Myc模块的基因表达水平不仅是总体上的相似，而且即使在比较单个基因的表达时，也有明显的相似之处。我们还观察到诱导多能干细胞(IPSCs)和部分IPSCs在单个Myc模块基因表达上的相似程度，部分IPSCs是IPSC诱导过程中产生的有害副产品。此外，我们的数据表明，部分IPSCs的自我更新特性依赖于高水平的c-Myc表达。
Predominant transcriptional subnetworks called Core, Myc, and PRC modules have been shown to participate in preservation of the pluripotency and self-renewality of embryonic stem cells (ESCs). Epiblast stem cells (EpiSCs) are another cell type that possesses pluripotency and self-renewality. However, the roles of these modules in EpiSCs have not been systematically examined to date. Here, we compared the average expression levels of Core, Myc, and PRC module genes between ESCs and EpiSCs. EpiSCs showed substantially higher and lower expression levels of PRC and Core module genes, respectively, compared with those in ESCs, while Myc module members showed almost equivalent levels of average gene expression. Subsequent analyses revealed that the similarity in gene expression levels of the Myc module between these two cell types was not just overall, but striking similarities were evident even when comparing the expression of individual genes. We also observed equivalent levels of similarity in the expression of individual Myc module genes between induced pluripotent stem cells (iPSCs) and partial iPSCs that are an unwanted byproduct generated during iPSC induction. Moreover, our data demonstrate that partial iPSCs depend on a high level of c-Myc expression for their self-renewal properties.
登录
查看更多内容
影响因子：
64.5
作者：
Kim J;Woo AJ;Chu J;Snow JW;Fujiwara Y;Kim CG;Cantor AB;Orkin SH
通讯作者：
Orkin SH
影响因子：
23.9
作者：
Ko, Kinarm;Tapia, Natalia;Schoeler, Hans R.
通讯作者：
Schoeler, Hans R.
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
64.8
作者：
Brons, I. Gabrielle M.;Smithers, Lucy E.;Vallier, Ludovic
通讯作者：
Vallier, Ludovic
影响因子：
64.5
作者：
Kim, Jonghwan;Chu, Jianlin;Orkin, Stuart H.
通讯作者：
Orkin, Stuart H.