Comparative Metabolomics Analysis of Cervicitis in Human Patients and a Phenol Mucilage-Induced Rat Model Using Liquid Chromatography Tandem Mass Spectrometry.
Comparative Metabolomics Analysis of Cervicitis in Human Patients and a Phenol Mucilage-Induced Rat Model Using Liquid Chromatography Tandem Mass Spectrometry.
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使用液相色谱串联质谱法对人类患者宫颈炎和苯酚粘液诱导的大鼠模型进行比较代谢组学分析
DOI：
10.3389/fphar.2018.00282
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发表时间：
2018
影响因子：
5.6
通讯作者：
Yang S
中科院分区：
文献类型：
--
作者：
Zhang X;Li J;Xie B;Wu B;Lei S;Yao Y;He M;Ouyang H;Feng Y;Xu W;Yang S
宫颈炎是一种非常常见的妇科疾病，它使妇女处于性传播感染的高危状态，并诱发一系列生殖系统疾病。这种情况也有显着的影响生活质量，并在临床实践中，由于其复杂的发病机制，往往被误诊。在本研究中，我们进行了非靶向血浆代谢组学分析宫颈炎的血浆样本从人类患者和血浆样本从苯酚胶浆诱导的大鼠模型的宫颈炎，使用超高效液相色谱耦合四极杆飞行时间串联质谱。除了在组织病理学的差异，我们确定了宫颈炎和对照组之间的代谢谱的差异，使用无监督的主成分分析和正交投影的潜在结构判别分析。这些结果证明了血浆代谢物的变化，在大鼠和人样本中分别鉴定出27种和22种潜在内源性标志物。代谢途径分析表明，亚油酸、花生四烯酸、醚脂和甘油磷脂代谢是宫颈炎的关键代谢途径。本研究成功地建立了大鼠宫颈炎模型，并应用于宫颈炎发病机制的研究。
Cervicitis is an exceedingly common gynecological disorder that puts women at high risk of sexually transmitted infections and induces a series of reproductive system diseases. This condition also has a significant impact on quality of life and is commonly misdiagnosed in clinical practice due to its complicated pathogenesis. In the present study, we performed non-targeted plasma metabolomics analysis of cervicitis in both plasma samples obtained from human patients and plasma samples from a phenol mucilage induced rat model of cervicitis, using ultra-performance liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry. In addition to differences in histopathology, we identified differences in the metabolic profile between the cervicitis and control groups using unsupervised principal component analysis and orthogonal projections to latent structures discriminant analysis. These results demonstrated changes in plasma metabolites, with 27 and 22 potential endogenous markers identified in rat and human samples, respectively. The metabolic pathway analysis showed that linoleic acid, arachidonic acid, ether lipid, and glycerophospholipid metabolism are key metabolic pathways involved in cervicitis. This study showed the rat model was successfully created and applied to understand the pathogenesis of cervicitis.
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