Circulating microRNA: The Potential Novel Diagnostic Biomarkers to Predict Drug Resistance in Temporal Lobe Epilepsy, a Pilot Study.
Circulating microRNA: The Potential Novel Diagnostic Biomarkers to Predict Drug Resistance in Temporal Lobe Epilepsy, a Pilot Study.
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DOI：
10.3390/ijms22020702
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发表时间：
2021-01-12
影响因子：
5.6
通讯作者：
Gambardella A
中科院分区：
文献类型：
--
作者：
De Benedittis S;Fortunato F;Cava C;Gallivanone F;Iaccino E;Caligiuri ME;Castiglioni I;Bertoli G;Manna I;Labate A;Gambardella A
MicroRNA (miRNA) 是小型非编码 RNA，已成为新的潜在表观遗传生物标志物。在这里，我们评估了文献中先前描述的六种循环 miRNA 的功效，这些 miRNA 作为诊断颞叶癫痫 (TLE) 的生物标志物和/或作为抗癫痫药物反应的预测生物标志物。我们通过定量逆转录聚合酶链反应 (qRT-PCR) 测定，测量了 27 名接受 TLE 治疗的患者（14 名女性和 13 名男性；平均值 ± SD 年龄：43.65 ± 17.07）与 20 名性别、年龄和种族匹配的健康对照 (HC)（11 名女性和 9 名男性；平均值 ± SD 年龄：47.5 ± 9.1）的血清 miRNA 水平的差异。此外，根据患者是否患有药物反应性（n = 17）或耐药性（n = 10）TLE 对患者进行分类。我们研究了 miRNA 和几个电临床参数之间的相关性。三种 miRNA（miR-142、miR-146a、miR-223）在患者中显着上调（表示为平均表达量±SD）。具体而言，与 HC 相比，TLE 患者中 miR-142 表达为 0.40 ± 0.29 vs. 0.16 ± 0.10（t 检验，p < 0.01），miR-146a 表达为 0.15 ± 0.11 vs. 0.07 ± 0.04（t 检验，p < 0.05），miR-223 表达为6.21 ± 3.65 与 1.23 ± 0.84（t 检验，p < 0.001）。此外，逻辑回归模型获得的结果显示 miR-142 和 miR-223 在区分药物敏感与耐药 TLE 方面具有良好的性能。这项初步研究的结果证明 miRNA 是 TLE 的合适靶点，并为更大的癫痫队列中的进一步确认研究提供了理论依据。
MicroRNAs (miRNAs) are small noncoding RNAs that have emerged as new potential epigenetic biomarkers. Here, we evaluate the efficacy of six circulating miRNA previously described in the literature as biomarkers for the diagnosis of temporal lobe epilepsy (TLE) and/or as predictive biomarkers to antiepileptic drug response. We measured the differences in serum miRNA levels by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) assays in a cohort of 27 patients (14 women and 13 men; mean ± SD age: 43.65 ± 17.07) with TLE compared to 20 healthy controls (HC) matched for sex, age and ethnicity (11 women and 9 men; mean ± SD age: 47.5 ± 9.1). Additionally, patients were classified according to whether they had drug-responsive (n = 17) or drug-resistant (n = 10) TLE. We have investigated any correlations between miRNAs and several electroclinical parameters. Three miRNAs (miR-142, miR-146a, miR-223) were significantly upregulated in patients (expressed as average expression ± SD). In detail, miR-142 expression was 0.40 ± 0.29 vs. 0.16 ± 0.10 in TLE patients compared to HC (t-test, p < 0.01), miR-146a expression was 0.15 ± 0.11 vs. 0.07 ± 0.04 (t-test, p < 0.05), and miR-223 expression was 6.21 ± 3.65 vs. 1.23 ± 0.84 (t-test, p < 0.001). Moreover, results obtained from a logistic regression model showed the good performance of miR-142 and miR-223 in distinguishing drug-sensitive vs. drug-resistant TLE. The results of this pilot study give evidence that miRNAs are suitable targets in TLE and offer the rationale for further confirmation studies in larger epilepsy cohorts.
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DOI：
10.1016/j.omtn.2018.09.008
发表时间：
2018-12-07
期刊：
Molecular therapy. Nucleic acids
影响因子：
--
作者：
Cava C;Manna I;Gambardella A;Bertoli G;Castiglioni I
通讯作者：
Castiglioni I
microRNA 作为癫痫生物标志物的挑战。
影响因子：
5.3
作者：
Ma Y
通讯作者：
Ma Y
影响因子：
5.6
作者：
Cava C;Colaprico A;Bertoli G;Graudenzi A;Silva TC;Olsen C;Noushmehr H;Bontempi G;Mauri G;Castiglioni I
通讯作者：
Castiglioni I
影响因子：
6.7
作者：
Dombkowski, Alan A.;Cukovic, Daniela;Chugani, Diane C.
通讯作者：
Chugani, Diane C.
影响因子：
3
作者：
Enright, Noelle;Simonato, Michele;Henshall, David C
通讯作者：
Henshall, David C