High-frequency near-infrared diode laser irradiation suppresses IL-1β-induced inflammatory cytokine expression and NF-κB signaling pathways in human primary chondrocytes
High-frequency near-infrared diode laser irradiation suppresses IL-1β-induced inflammatory cytokine expression and NF-κB signaling pathways in human primary chondrocytes
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高频近红外二极管激光照射抑制人原代软骨细胞中 IL-1β 诱导的炎症细胞因子表达和 NF-κB 信号通路
DOI：
10.1007/s10103-021-03371-5
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Tanimoto K.
中科院分区：
文献类型：
--
作者：
Sakata S.;Kunimastu R.;Tsuka Y.;Nakatani S.;Gunji H.;Yanoshita M.;Kado I.;Ito S.;Putranti NAR.;RC Prasetya.;Hirose N.;Tanimoto K.
骨关节炎(OA)和类风湿关节炎(RA)是常见的炎症相关性软骨退行性疾病。最近的研究表明，低强度半导体激光治疗可以减少炎症细胞因子在软骨中的表达。我们最近报道了高频低强度半导体激光照射对人原代软骨细胞基质金属蛋白酶(MMPs)表达的影响。然而，高频低强度半导体激光对软骨细胞作用的分子机制尚不清楚。因此，我们旨在阐明高频低强度半导体激光照射对人原代软骨细胞炎性细胞因子表达的影响。用重组白介素1β(IL-1β)处理正常人关节软骨细胞30h或24h，用功率为8J/cm~2的高频近红外半导体激光照射软骨细胞。免疫印迹法检测IL-1β、IL-6和肿瘤坏死因子-α的表达。为探讨核因子-kappaB(NF-κB)信号转导途径，采用免疫印迹分析、免疫荧光分析、凝胶迁移率改变分析和酶联免疫吸附分析等方法检测核因子-kappaB的磷酸化、易位和κ结合活性。高频低强度半导体激光照射可降低IL-1β诱导的软骨细胞炎性细胞因子的表达。此外，高频低强度半导体激光照射降低了IL-1κ处理状态下的NF-βB的磷酸化、核转位和DNA结合活性。然而，单独照射并不影响NF-κB的活性。因此，8J/cm2的高频低强度半导体激光照射可通过对核因子-κB的调节，减少正常关节软骨细胞炎性细胞因子的表达。这些结果表明，高频低强度半导体激光照射可以减少炎性细胞因子在OA和RA中的表达。
Osteoarthritis (OA) and rheumatoid arthritis (RA) are common inflammation-associated cartilage degenerative diseases. Recent studies have shown that low-level diode laser treatment can reduce inflammatory cytokine expressions in cartilage. We recently reported that high-frequency low-level diode laser irradiation attenuates matrix metalloproteinases (MMPs) expression in human primary chondrocytes. However, the molecular mechanism underlying the effect of high-frequency low-level diode laser on chondrocytes remains unclear. Therefore, we aimed to elucidate the effect of high-frequency low-level diode laser irradiation on inflammatory cytokine expression in human primary chondrocytes. Normal human articular chondrocytes were treated with recombinant interleukin-1 beta (IL-1β) for 30 min or 24 h and irradiated with a high-frequency NIR diode laser at 8 J/cm2. The expression of IL-1β, interleukin-6, and tumor necrosis factor-alpha was assessed using western blot analysis. To evaluate the nuclear factor-kappa B (NF-κB) signaling pathway, the phosphorylation, translocation, and DNA-binding activity of NF-κB were detected using western blot analysis, immunofluorescence analysis, electrophoretic mobility shift assay, and enzyme-linked immunosorbent assay analysis. High-frequency low-level diode laser irradiation decreased inflammatory cytokine expression in IL-1β-treated chondrocytes. Moreover, high-frequency low-level diode laser irradiation decreased the phosphorylation, nuclear translocation, and DNA-binding activity of NF-κB in the IL-1β-treated state. However, irradiation alone did not affect NF-κB activity. Thus, high-frequency low-level diode laser irradiation at 8 J/cm2can reduce inflammatory cytokine expressions in normal human articular chondrocytes through NF-κB regulation. These findings indicate that high-frequency low-level diode laser irradiation may reduce the expression of inflammatory cytokines in OA and RA.
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