Enteroendocrine cells are specifically marked by cell surface expression of claudin-4 in mouse small intestine.
Enteroendocrine cells are specifically marked by cell surface expression of claudin-4 in mouse small intestine.
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DOI：
10.1371/journal.pone.0090638
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Hamazaki Y
中科院分区：
文献类型：
--
作者：
Nagatake T;Fujita H;Minato N;Hamazaki Y
肠内分泌细胞是分散在胃肠道各处的孤立上皮细胞，产生各种激素，是人体最大的内分泌系统之一。由于缺乏特异性的细胞表面标记物，这些罕见上皮细胞的分离困难，阻碍了对它们的研究。在这里，我们报告了肠内分泌细胞选择性表达紧密连接膜蛋白Cld4 (Cld4)，并使用Cld4细胞外结构域特异性抗体和流式细胞术有效地分离。经筛选的小肠Cld4+上皮细胞只表达一个嗜铬粒蛋白a基因（Chga）和其他肠内分泌细胞相关基因（Ffar1、Ffar4、Gpr119），并根据与欧洲巨噬细胞凝集素-1 （UEA-1）的结合活性将群体分为两个亚群。Cld4+UEA-1细胞群几乎完全表达葡萄糖依赖性胰岛素性多肽基因（Gip），因此代表K细胞，而Cld4+UEA-1细胞群表达其他肠道激素基因，包括胰高血糖素样肽1 (Gcg)，胰多肽样肽与n端酪氨酸酰胺（Pyy），胆囊收缩素（Cck），分泌素（Sct）和色氨酸羟化酶1 （Tph1）。此外，我们发现口服给药的腔内抗原被小肠绒毛中的孤立Cld4+细胞吸收，这提高了肠内分泌细胞也可能在粘膜免疫启动中发挥作用的可能性。我们的结果为肠内分泌细胞的细胞和功能表征提供了有用的工具。
Enteroendocrine cells are solitary epithelial cells scattered throughout the gastrointestinal tract and produce various types of hormones, constituting one of the largest endocrine systems in the body. The study of these rare epithelial cells has been hampered by the difficulty in isolating them because of the lack of specific cell surface markers. Here, we report that enteroendocrine cells selectively express a tight junction membrane protein, claudin-4 (Cld4), and are efficiently isolated with the use of an antibody specific for the Cld4 extracellular domain and flow cytometry. Sorted Cld4+ epithelial cells in the small intestine exclusively expressed a chromogranin A gene (Chga) and other enteroendocrine cell–related genes (Ffar1, Ffar4, Gpr119), and the population was divided into two subpopulations based on the activity of binding to Ulex europaeus agglutinin-1 (UEA-1). A Cld4+UEA-1− cell population almost exclusively expressed glucose-dependent insulinotropic polypeptide gene (Gip), thus representing K cells, whereas a Cld4+UEA-1+ cell population expressed other gut hormone genes, including glucagon-like peptide 1 (Gcg), pancreatic polypeptide–like peptide with N-terminal tyrosine amide (Pyy), cholecystokinin (Cck), secretin (Sct), and tryptophan hydroxylase 1 (Tph1). In addition, we found that orally administered luminal antigens were taken up by the solitary Cld4+ cells in the small intestinal villi, raising the possibility that enteroendocrine cells might also play a role in initiation of mucosal immunity. Our results provide a useful tool for the cellular and functional characterization of enteroendocrine cells.
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影响因子：
4.8
作者：
Habib, Abdella M.;Richards, Paul;Gribble, Fiona M.
通讯作者：
Gribble, Fiona M.
DOI：
10.1111/j.1749-6632.2012.06555.x
发表时间：
2012-01-01
期刊：
BARRIERS AND CHANNELS FORMED BY TIGHT JUNCTION PROTEINS I
影响因子：
--
作者：
Krug, Susanne M.;Guenzel, Dorothee;Yu, Alan S. L.
通讯作者：
Yu, Alan S. L.
影响因子：
3.7
作者：
Fujita H;Hamazaki Y;Noda Y;Oshima M;Minato N
通讯作者：
Minato N
影响因子：
4.8
作者：
Chu, Zhi-Liang;Carroll, Chris;Leonard, James
通讯作者：
Leonard, James
影响因子：
2.4
作者：
MADARA, JL;TRIER, JS
通讯作者：
TRIER, JS