Dissemination of NDM-1-Producing Enterobacteriaceae Mediated by the IncX3-Type Plasmid.
Dissemination of NDM-1-Producing Enterobacteriaceae Mediated by the IncX3-Type Plasmid.
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IncX3 型质粒介导的产 NDM-1 肠杆菌科细菌的传播
DOI：
10.1371/journal.pone.0129454
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Yu Y
中科院分区：
文献类型：
--
作者：
Yang Q;Fang L;Fu Y;Du X;Shen Y;Yu Y
产生NDM-1的肠杆菌科细菌的出现和传播已经造成了全球性的公共卫生风险，已经影响到中国的一些省份。中国是一个幅员辽阔的国家，迫切需要调查我省BLANDM-1阳性肠杆菌科细菌的流行情况。从浙江省某三甲医院分离到186株耐碳青霉烯类肠杆菌科细菌(CRE)。对碳青霉烯类耐药基因bla kpc、bla imp、bla vim、bla OXA-48和bla NDM-1进行筛选和测序。经鉴定，90株BLAKPC-2基因阳性，5株BLANDM-1阳性。XbaI-PFGE结果显示，3株BLANDM-1阳性肺炎克雷伯菌分属两个不同的克隆。S_1-pFGE和Southern杂交结果表明，bla-ndm-1基因定位于大小分别为33.3kb~54.7kb(n=4)和104.5 kb~138.9 kb(n=1)的IncX3型载体上，均可通过接合和电转化的方式转移到大肠杆菌中。对两个质粒进行高通量测序后，鉴定出一个较小的54kb质粒，它与先前报道的pCFNDM-CN具有很高的序列相似性，以及一个较大的质粒，其中只检测到bla NDM-1周围共同基因环境的7.8kb序列(bla NDM-1-trpF-dsbC-utA1-GroEL-Δinse)。PCR定位和测序表明，4个较小的bla NDM-1质粒在bla NDM-1周围含有共同的基因环境(is5-bla NDM-1-trpF-dsbC-utA1-GroEL)。我们监测了本院CRE的流行情况，确定KPC-2碳青霉烯酶是对患者健康的主要威胁，而IncX3型质粒在CRE中起着至关重要的作用。
The emergence and spread of NDM-1-producing Enterobacteriaceae have resulted in a worldwide public health risk that has affected some provinces of China. China is an exceptionally large country, and there is a crucial need to investigate the epidemic of bla NDM-1-positive Enterobacteriaceae in our province. A total of 186 carbapenem-resistant Enterobacteriaceae isolates (CRE) were collected in a grade-3 hospital in Zhejiang province. Carbapenem-resistant genes, including bla KPC, bla IMP, bla VIM, bla OXA-48 and bla NDM-1 were screened and sequenced. Ninety isolates were identified as harboring the bla KPC-2 genes, and five bla NDM-1-positive isolates were uncovered. XbaI-PFGE revealed that three bla NDM-1-positive K. pneumoniae isolates belonged to two different clones. S1-PFGE and southern blot suggested that the bla NDM-1 genes were located on IncX3-type plasmids with two different sizes ranging from 33.3 to 54.7 kb (n=4) and 104.5 to 138.9 kb (n=1), respectively, all of which could easily transfer to Escherichia coli by conjugation and electrotransformation. The high-throughput sequencing of two plasmids was performed leading to the identification of a smaller 54-kb plasmid, which had high sequence similarity with a previously reported pCFNDM-CN, and a larger plasmid in which only a 7.8-kb sequence of a common gene environment around bla NDM-1 (bla NDM-1-trpF- dsbC-cutA1-groEL-ΔInsE,) was detected. PCR mapping and sequencing demonstrated that four smaller bla NDM-1 plasmids contained a common gene environment around bla NDM-1 (IS5-bla NDM-1-trpF- dsbC-cutA1-groEL). We monitored the CRE epidemic in our hospital and determined that KPC-2 carbapenemase was a major risk to patient health and the IncX3-type plasmid played a vital role in the spread of the bla NDM-1 gene among the CRE.
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影响因子：
5.2
作者：
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通讯作者：
Carattoli, Alessandra
影响因子：
11.8
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影响因子：
4.9
作者：
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影响因子：
9.4
作者：
Doyle, Diana;Peirano, Gisele;Pitout, Johann D. D.
通讯作者：
Pitout, Johann D. D.
影响因子：
13.2
作者：
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通讯作者：
Chow, Kin-Hung