Identification and characterization of a novel incompatibility group X3 plasmid carrying bla NDM-1 in Enterobacteriaceae isolates with epidemiological links to multiple geographical areas in China.
Identification and characterization of a novel incompatibility group X3 plasmid carrying bla NDM-1 in Enterobacteriaceae isolates with epidemiological links to multiple geographical areas in China.
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DOI：
10.1038/emi.2012.37
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发表时间：
2012-11
影响因子：
13.2
通讯作者：
Chow, Kin-Hung
中科院分区：
文献类型：
--
作者：
Ho, Pak-Leung;Li, Zhen;Lo, Wai-U;Cheung, Yuk-Yam;Lin, Chi-Ho;Sham, Pak-Chung;Cheng, Vincent Chi-Chung;Ng, Tak-Keung;Que, Tak-Lun;Chow, Kin-Hung
新德里金属β-内酰胺酶（NDM-1）是肠杆菌科细菌最重要的耐药特性之一。我们表征了2010年12月至2012年5月期间从最近在印度（n=1）或中国大陆（n=6）旅行或接受治疗的7名患者中回收的9株产blaNDM-1肠杆菌。所有与中国有关的患者都与印度次大陆没有联系。blaNDM-1质粒在大肠埃希菌、肺炎克雷伯菌、弗氏柠檬酸杆菌、产气肠杆菌和大肠埃希菌等7株菌中均属于新的IncX 3（约50 kb）。clopidin）、印加/C2（约140 kb），在一株E. coli）或FII-F1 B群（约110 kb，在一个E.大肠杆菌）。7个IncX 3质粒的限制性片段长度多态性分析显示，在六个相同的模式和两个带的差异，在其余的一个。携带blaNDM-1的IncX 3质粒与中国大陆的广州（n=1）、湖南（n=4）、海丰（n=1）和东莞（n=1）流行病学连锁。测序结果表明，IncX 3质粒pNDM-HN 380全长54 035 bp，编码52个开放阅读框。发现blaNDM-1基因位于转座子样结构中，侧翼为ISAba 125和IS 26，插入质粒遗传负载区。两个IS元件中含有blaNDM-1的模块的序列与先前描述的不动杆菌属分离株质粒或染色体中blaNDM-1阳性Tn 125的序列相同。总之，这是首次描述携带blaNDM-1的IncX 3质粒。研究结果表明，令人担忧的是，流行性质粒参与了NDM-1在中国的传播。
The New Delhi metallo-β-lactamase (NDM-1) is one of the most important resistance traits in Enterobacteriaceae. We characterized nine blaNDM-1 producing Enterobacteriaceae recovered from seven patients who have recently travelled or been treated in India (n=1) or mainland China (n=6) during December 2010–May 2012. All the China-linked patients had no links to the Indian subcontinent. The blaNDM-1 carrying plasmids belonged to the novel IncX3 (∼50 kb, in seven isolates including two Escherichia coli, two Klebsiella pneumoniae, one Citrobacter freundii, one Enterobacter aerogenes and one E. cloacae), IncA/C2 (∼140 kb, in one E. coli) or FII-F1B groups (∼110 kb, in one E. coli). Restriction fragment length polymorphism analysis of the seven IncX3 plasmids revealed identical pattern in six and two bands difference in the remaining one. The IncX3 plasmids carrying blaNDM-1 were epidemiologically linked to Guangzhou (n=1), Hunan (n=4), Haifeng (n=1) and Dongguan (n=1) in mainland China. Complete sequencing of the IncX3 plasmid pNDM-HN380 revealed that it was 54 035 bp long and encoded 52 open reading frames. The blaNDM-1 gene was found in a transposon-like structure flanked by ISAba125 and IS26, inserted into the plasmid genetic load region. The sequences of the blaNDM-1 containing module within the two IS elements were identical to those previously described for blaNDM-1-positive Tn125 in the plasmids or chromosome of Acinetobacter isolates. In summary, this is the first description of IncX3 plasmids carrying blaNDM-1. The findings indicate the worrisome involvement of an epidemic plasmid in the dissemination of NDM-1 in China.
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